9QFW
Cryo-EM structure of the cofilactin barbed end bound by two AIP1 molecules
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E (A, B, C, D, E) | Actin, cytoplasmic 1, N-terminally processed | polymer | 374 | 41632.4 | 5 | UniProt (P60709) Pfam (PF00022) | Homo sapiens (human) | |
| 2 | F, G, H, I, J (F, G, H, I, J) | Cofilin-1 | polymer | 166 | 18532.5 | 5 | UniProt (P23528) Pfam (PF00241) | Homo sapiens (human) | 18 kDa phosphoprotein,p18,Cofilin,non-muscle isoform |
| 3 | K, L (P, Q) | WD repeat-containing protein 1,Methylated-DNA--protein-cysteine methyltransferase | polymer | 805 | 86760.9 | 2 | UniProt (O75083) UniProt (P16455) Pfam (PF00400) Pfam (PF02870) Pfam (PF01035) | Homo sapiens (human) | Actin-interacting protein 1,AIP1,NORI-1,6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase |
| 4 | M, O, Q, S, U (A, B, C, D, E) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | N, P, R, T, V (A, B, C, D, E) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 5 | Chemie (ADP) |
Sequence modifications
A, B, C, D, E: 2 - 375 (UniProt: P60709)
P, Q: 1 - 606 (UniProt: O75083)
P, Q: 619 - 800 (UniProt: P16455)
| PDB | External Database | Details |
|---|---|---|
| Ala 272 | Cys 272 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Met -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 607 | - | linker |
| Thr 608 | - | linker |
| Gly 609 | - | linker |
| Gly 610 | - | linker |
| Gly 611 | - | linker |
| Gly 612 | - | linker |
| Ser 613 | - | linker |
| Gly 614 | - | linker |
| Gly 615 | - | linker |
| Gly 616 | - | linker |
| Gly 617 | - | linker |
| Ser 618 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 680 | Cys 62 | conflict |
| Ala 743 | Lys 125 | conflict |
| Thr 745 | Ala 127 | conflict |
| Ala 746 | Arg 128 | conflict |
| Lys 749 | Gly 131 | conflict |
| Thr 750 | Gly 132 | conflict |
| Leu 752 | Met 134 | conflict |
| Ser 753 | Arg 135 | conflict |
| Ser 768 | Cys 150 | conflict |
| Gly 775 | Asn 157 | conflict |
| Glu 777 | Ser 159 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 474346.6 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2257.5 | |
| All* | Total formula weight | 476604.1 |
