9QF2
Cryo-EM structure of the fully Coronin-1B-decorated actin filament in the ADP state.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, D, E, F (C, A, B, D, E) | Actin, cytoplasmic 1, N-terminally processed | polymer | 374 | 41632.4 | 5 | UniProt (P60709) Pfam (PF00022) | Homo sapiens (human) | |
| 2 | B, G, H, I, J (H, F, G, I, J) | Coronin-1B,Methylated-DNA--protein-cysteine methyltransferase | polymer | 688 | 74788.1 | 5 | UniProt (Q9BR76) UniProt (P16455) Pfam (PF08953) Pfam (PF00400) Pfam (PF16300) Pfam (PF02870) Pfam (PF01035) | Homo sapiens (human) | Coronin-2,6-O-methylguanine-DNA methyltransferase,MGMT,O-6-methylguanine-DNA-alkyltransferase |
| 3 | K, M, O, Q, S (C, A, B, D, E) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 5 | Chemie (ADP) | |||
| 4 | L, N, P, R, T (C, A, B, D, E) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | AA, BA, CA, DA, U... (F, G, I, J, C...) | water | water | 18.0 | 672 | Chemie (HOH) |
Sequence modifications
C, A, B, D, E: 2 - 375 (UniProt: P60709)
H, F, G, I, J: 1 - 489 (UniProt: Q9BR76)
H, F, G, I, J: 502 - 683 (UniProt: P16455)
| PDB | External Database | Details |
|---|---|---|
| Ala 272 | Cys 272 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Met -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 490 | - | linker |
| Thr 491 | - | linker |
| Gly 492 | - | linker |
| Gly 493 | - | linker |
| Gly 494 | - | linker |
| Gly 495 | - | linker |
| Ser 496 | - | linker |
| Gly 497 | - | linker |
| Gly 498 | - | linker |
| Gly 499 | - | linker |
| Gly 500 | - | linker |
| Ser 501 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ala 563 | Cys 62 | conflict |
| Ala 626 | Lys 125 | conflict |
| Thr 628 | Ala 127 | conflict |
| Ala 629 | Arg 128 | conflict |
| Lys 632 | Gly 131 | conflict |
| Thr 633 | Gly 132 | conflict |
| Leu 635 | Met 134 | conflict |
| Ser 636 | Arg 135 | conflict |
| Ser 651 | Cys 150 | conflict |
| Gly 658 | Asn 157 | conflict |
| Glu 660 | Ser 159 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 582102.5 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 2257.5 | |
| All* | Total formula weight | 584360.1 |
