9QEQ
Structure of the transcribing Pol II-DSIF-SPT6-U1 snRNP complex
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA-directed RNA polymerase subunit | polymer | 1970 | 217450.1 | 1 | UniProt (A0A8D1DPV6) | Sus scrofa domesticus (domestic pig) | |
| 2 | B (B) | DNA-directed RNA polymerase subunit beta | polymer | 1174 | 134041.4 | 1 | UniProt (A0A0B8RVL1) | Sus scrofa domesticus (domestic pig) | |
| 3 | C (C) | DNA-directed RNA polymerase II subunit RPB3 | polymer | 275 | 31439.1 | 1 | UniProt (I3LCH3) Pfam (PF01193) Pfam (PF01000) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit C |
| 4 | D (D) | RNA polymerase II subunit D | polymer | 142 | 16331.3 | 1 | UniProt (A0A4X1VM56) Pfam (PF03874) UniProt (by SIFTS) (A0A2Y9PTG7) | Sus scrofa domesticus (domestic pig) | |
| 5 | E (E) | DNA-directed RNA polymerase II subunit E | polymer | 210 | 24644.3 | 1 | UniProt (I3LSI7) Pfam (PF03871) Pfam (PF01191) | Sus scrofa domesticus (domestic pig) | RPB5 homolog |
| 6 | F (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 127 | 14477.0 | 1 | UniProt (A0A4X1VEK9) Pfam (PF01192) | Sus scrofa domesticus (domestic pig) | DNA-directed RNA polymerase II subunit F,RPB6 homolog |
| 7 | G (G) | DNA-directed RNA polymerase subunit | polymer | 172 | 19314.3 | 1 | UniProt (A0A4X1VKG7) Pfam (PF03876) Pfam (PF00575) UniProt (by SIFTS) (A0A2Y9PKI8) | Sus scrofa domesticus (domestic pig) | |
| 8 | H (H) | DNA-directed RNA polymerases I, II, and III subunit RPABC3 | polymer | 150 | 17162.3 | 1 | UniProt (I3LCB2) Pfam (PF03870) UniProt (by SIFTS) (A0A6I8N3L3) | Sus scrofa domesticus (domestic pig) | |
| 9 | I (I) | DNA-directed RNA polymerase II subunit RPB9 | polymer | 125 | 14541.2 | 1 | UniProt (P60899) Pfam (PF02150) Pfam (PF01096) | Sus scrofa domesticus (domestic pig) | RNA polymerase II subunit B9,DNA-directed RNA polymerase II subunit I,RNA polymerase II 14.5 kDa subunit,RPB14.5 |
| 10 | J (J) | DNA-directed RNA polymerases I, II, and III subunit RPABC5 | polymer | 67 | 7655.1 | 1 | UniProt (A0A4X1VYD0) Pfam (PF01194) UniProt (by SIFTS) (A0A8W4F9W9) | Sus scrofa domesticus (domestic pig) | |
| 11 | K (K) | DNA-directed RNA polymerase II subunit RPB11-a | polymer | 117 | 13310.3 | 1 | UniProt (F1RKE4) Pfam (PF13656) UniProt (by SIFTS) (F7CZX7) | Sus scrofa domesticus (domestic pig) | |
| 12 | L (L) | RNA polymerase II, I and III subunit K | polymer | 58 | 7018.2 | 1 | UniProt (A0A4X1TRS6) Pfam (PF03604) | Sus scrofa domesticus (domestic pig) | |
| 13 | M (M) | Transcription elongation factor SPT6 | polymer | 1726 | 199330.7 | 1 | UniProt (Q7KZ85) Pfam (PF14632) Pfam (PF14641) Pfam (PF22706) Pfam (PF14639) Pfam (PF14635) Pfam (PF17674) Pfam (PF00575) Pfam (PF14633) | Homo sapiens (human) | hSPT6,Histone chaperone suppressor of Ty6,Tat-cotransactivator 2 protein,Tat-CT2 protein |
| 14 | N (N) | Non-template DNA | polymer | 48 | 14932.5 | 1 | synthetic construct | ||
| 15 | O (P) | Pre-mRNA | polymer | 68 | 21967.1 | 1 | synthetic construct | ||
| 16 | P (T) | Template DNA | polymer | 48 | 14672.3 | 1 | synthetic construct | ||
| 17 | Q (Y) | Transcription elongation factor SPT4 | polymer | 121 | 13508.5 | 1 | UniProt (Q4R941) Pfam (PF06093) | Homo sapiens (human) | DRB sensitivity-inducing factor small subunit,DSIF small subunit |
| 18 | R (Z) | Transcription elongation factor SPT5 | polymer | 1087 | 121145.5 | 1 | UniProt (O00267) Pfam (PF11942) Pfam (PF03439) Pfam (PF00467) Pfam (PF23037) Pfam (PF23288) Pfam (PF23287) | Homo sapiens (human) | hSPT5,DRB sensitivity-inducing factor 160 kDa subunit,DSIF p160,DRB sensitivity-inducing factor large subunit,DSIF large subunit,Tat-cotransactivator 1 protein,Tat-CT1 protein |
| 19 | S (a) | U1 snRNA | polymer | 164 | 52697.1 | 1 | GenBank (2795782232) | Homo sapiens (human) | |
| 20 | T (b) | U1 small nuclear ribonucleoprotein 70 kDa | polymer | 437 | 51683.2 | 1 | UniProt (P08621) Pfam (PF12220) Pfam (PF00076) | Homo sapiens (human) | U1 snRNP 70 kDa,U1-70K,snRNP70 |
| 21 | U (c) | U1 small nuclear ribonucleoprotein A | polymer | 282 | 31319.5 | 1 | UniProt (P09012) Pfam (PF00076) | Homo sapiens (human) | U1A |
| 22 | V (e) | Small nuclear ribonucleoprotein Sm D2 | polymer | 118 | 13551.9 | 1 | UniProt (P62316) Pfam (PF01423) | Homo sapiens (human) | Sm-D2,snRNP core protein D2 |
| 23 | W (f) | Small nuclear ribonucleoprotein F | polymer | 86 | 9734.2 | 1 | UniProt (P62306) Pfam (PF01423) | Homo sapiens (human) | snRNP-F,Sm protein F,SmF |
| 24 | X (g) | Small nuclear ribonucleoprotein E | polymer | 92 | 10817.6 | 1 | UniProt (P62304) Pfam (PF01423) | Homo sapiens (human) | snRNP-E,Sm protein E,SmE |
| 25 | Y (h) | Small nuclear ribonucleoprotein G | polymer | 76 | 8508.1 | 1 | UniProt (P62308) Pfam (PF01423) | Homo sapiens (human) | snRNP-G,Sm protein G,SmG |
| 26 | Z (i) | Small nuclear ribonucleoprotein Sm D3 | polymer | 126 | 13940.3 | 1 | UniProt (P62318) Pfam (PF01423) | Homo sapiens (human) | Sm-D3,snRNP core protein D3 |
| 27 | AA (j) | Small nuclear ribonucleoprotein-associated protein | polymer | 231 | 23686.0 | 1 | UniProt (Q66K91) | Homo sapiens (human) | |
| 28 | BA (k) | Small nuclear ribonucleoprotein Sm D1 | polymer | 119 | 13310.7 | 1 | UniProt (P62314) Pfam (PF01423) | Homo sapiens (human) | Sm-D1,Sm-D autoantigen,snRNP core protein D1 |
| 29 | CA, DA, FA, GA, HA... (A, B, C, I, J...) | ZINC ION | non-polymer | 65.4 | 8 | Chemie (ZN) | |||
| 30 | EA (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
Y: 5 - 121 (UniProt: Q4R941)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Pro 2 | - | expression tag |
| Gly 3 | - | expression tag |
| Ser 4 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 28 |
| Total formula weight | 1132189.8 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 547.6 | |
| All* | Total formula weight | 1132737.4 |
