9QDV

Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant expressed in E. coli (E_PHL7mut3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Polyester Hydrolase Leipzig 7 (PHL-7), catalysis-deficient S131A mutant	polymer	267	29059.3	2	UniProt (A0AA82WPD4) Pfam (PF03403) UniProt (by SIFTS) (A0A918GGJ7)	compost metagenome
2	C, D (A, B)	water	water		18.0	840	Chemie (HOH)

Sequence modifications

A, B: 1 - 259 (UniProt: A0AA82WPD4)

PDB	External Database	Details
Ser 131	Ala 131	engineered mutation
Glu 175	Gln 175	engineered mutation
Thr 210	Leu 210	engineered mutation
Lys 233	Asp 233	engineered mutation
Leu 260	-	expression tag
Glu 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag
His 266	-	expression tag
His 267	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	58118.6
All*	Total formula weight	58118.6

*Water molecules are not included.