9QDE

Crystal structure of the non-glycosylated polyester hydrolase Leipzig 7 (PHL7) mut3 variant expressed in Pichia pastoris (P_PHL7mut3_ng)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polyester Hydrolase Leipzig 7 (PHL-7), catalysis-deficient S131A mutant	polymer	264	28727.9	1	UniProt (A0AA82WPD4) Pfam (PF12740) UniProt (by SIFTS) (A0A918GGJ7)	compost metagenome
2	B (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL) PubChem (312)
3	C (A)	water	water		18.0	443	Chemie (HOH)

Sequence modifications

A: 2 - 259 (UniProt: A0AA82WPD4)

PDB	External Database	Details
Ser 131	Ala 131	engineered mutation
Gln 143	Asn 143	engineered mutation
Gln 144	Asn 144	engineered mutation
Gln 161	Asn 161	engineered mutation
Glu 175	Gln 175	engineered mutation
Thr 210	Leu 210	engineered mutation
Lys 233	Asp 233	engineered mutation
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28727.9
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	28763.4

*Water molecules are not included.