9QCC
Activated XauSPARDA filament assembly with bound dsDNA substrate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, CA, E, I, M... (A, E, I, M, Q...) | DUF4365 domain-containing protein | polymer | 458 | 52420.3 | 8 | UniProt (A7ICE8) Pfam (PF14280) | Xanthobacter autotrophicus Py2 | |
| 2 | B, DA, F, J, N... (B, F, J, N, R...) | Protein argonaute | polymer | 482 | 53589.7 | 8 | UniProt (A7ICE9) | Xanthobacter autotrophicus Py2 | |
| 3 | AA, C, EA, G, K... (C, G, K, O, 1...) | DNA (5'-D(*AP*TP*TP*CP*GP*GP*CP*CP*GP*TP*GP*TP*AP*CP*AP*AP*T)-3') | polymer | 17 | 5202.4 | 8 | Escherichia coli | ||
| 4 | BA, D, FA, H, L... (D, H, L, P, 2...) | RNA (5'-R(P*AP*UP*UP*GP*UP*AP*CP*AP*CP*GP*GP*CP*CP*GP*AP*AP*U)-3') | polymer | 17 | 5427.3 | 8 | Escherichia coli | ||
| 5 | GA (S) | dsDNA substrate, first strand | polymer | 42 | 12994.3 | 1 | Escherichia coli | ||
| 6 | HA (T) | dsDNA substrate, second strand | polymer | 42 | 12862.3 | 1 | Escherichia coli | ||
| 7 | IA, JA, KA, LA, MA... (A, B, F, J, N...) | CALCIUM ION | non-polymer | 40.1 | 12 | Chemie (CA) |
Sequence modifications
A, E, I, M, Q, U, W, Y: 1 - 444 (UniProt: A7ICE8)
| PDB | External Database | Details |
|---|---|---|
| Met -13 | - | initiating methionine |
| Gly -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| His -8 | - | expression tag |
| His -7 | - | expression tag |
| His -6 | - | expression tag |
| His -5 | - | expression tag |
| His -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Met -2 | - | expression tag |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 34 |
| Total formula weight | 958973.4 | |
| Non-Polymers* | Number of molecules | 12 |
| Total formula weight | 480.9 | |
| All* | Total formula weight | 959454.3 |
