9QBN

Crystal structure of the polyester hydrolase Leipzig 7 (PHL7) mut3 variant with glycosylation by expression in Pichia pastoris (P_PHL7mut3)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Polyester Hydrolase Leipzig 7 (PHL-7), catalysis-deficient S131A mutant	polymer	265	28817.0	1	UniProt (A0AA82WPD4)	compost metagenome
2	B (B)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
3	C, D (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	E (A)	water	water		18.0	445	Chemie (HOH)

Sequence modifications

A: 1 - 259 (UniProt: A0AA82WPD4)

PDB	External Database	Details
Ser 131	Ala 131	engineered mutation
Glu 175	Gln 175	engineered mutation
Thr 210	Leu 210	engineered mutation
Lys 233	Asp 233	engineered mutation
His 260	-	expression tag
His 261	-	expression tag
His 262	-	expression tag
His 263	-	expression tag
His 264	-	expression tag
His 265	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	28817.0
Branched	Number of molecules	1
	Total formula weight	424.4
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	29433.5

*Water molecules are not included.