9QBJ

Legobody dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (C, D)	Nanobody ALFA-H6	polymer	133	14813.5	2		Vicugna pacos
2	C, E (E, G)	Fab antibody 8D3_2_H-H6	polymer	237	25539.6	2		Mus musculus
3	D, F (F, H)	Fa antibody 8D3_2_L	polymer	219	24095.9	2		Mus musculus
4	G (I)	Maltose/maltodextrin-binding periplasmic protein,Immunoglobulin G-binding protein A,Immunoglobulin G-binding protein G	polymer	543	59047.1	1	UniProt (P0AEY0) UniProt (P02976) UniProt (P99134) UniProt (P19909) Pfam (PF17573) Pfam (PF01378) Pfam (PF02216)	Escherichia coli	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,IgG-binding protein A,Staphylococcal protein A,SpA,IgG-binding protein G
5	H (J)	Maltose/maltodextrin-binding periplasmic protein,Immunoglobulin G-binding protein A,Immunoglobulin G-binding protein G	polymer	544	59176.2	1	UniProt (P99134) UniProt (P19909) UniProt (P0AEY0) UniProt (P02976) Pfam (PF17573) Pfam (PF01378) Pfam (PF02216)	Escherichia coli	MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP,IgG-binding protein A,Staphylococcal protein A,SpA,IgG-binding protein G
6	I, J (A, B)	alpha-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		342.3	2	In PDB GlyTouCan (G66120GK)

Sequence modifications

I: 10 - 367 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Gln 368	-	linker
Ala 369	-	linker
Leu 370	-	linker
Ala 371	-	linker
Phe 372	-	linker
Ala 373	-	linker
Gln 374	-	linker
Ile 375	-	linker
Leu 376	-	linker
Ile 377	-	linker
Met 378	-	linker
Pro 379	-	linker
Asn 380	-	linker
Leu 381	-	linker
Thr 382	-	linker
Glu 383	-	linker
Glu 384	-	linker

I: 385 - 411 (UniProt: P02976)

PDB	External Database	Details
Glu 412	-	linker
His 413	-	linker
Gln 414	-	linker
Ala 415	-	linker
Pro 416	-	linker
Lys 417	-	linker
Gly 418	-	linker
Gly 419	-	linker
Ser 420	-	linker
Gly 421	-	linker
Gly 422	-	linker
Ala 423	-	linker
Gly 424	-	linker
Ser 425	-	linker
Gly 426	-	linker

I: 427 - 475 (UniProt: P99134)

PDB	External Database	Details
Gly 476	-	linker
Gly 477	-	linker
Gly 478	-	linker
Ser 479	-	linker
Gly 480	-	linker
Gly 481	-	linker
Gly 482	-	linker
Ser 483	-	linker
Gly 484	-	linker
Gly 485	-	linker
Ser 486	-	linker

I: 487 - 543 (UniProt: P19909)
J: 10 - 367 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
Gly 8	-	expression tag
Ser 9	-	expression tag
Gln 368	-	linker
Ala 369	-	linker
Leu 370	-	linker
Ala 371	-	linker
Phe 372	-	linker
Ala 373	-	linker
Gln 374	-	linker
Ile 375	-	linker
Leu 376	-	linker
Ile 377	-	linker
Met 378	-	linker
Pro 379	-	linker
Asn 380	-	linker
Leu 381	-	linker
Thr 382	-	linker
Glu 383	-	linker
Glu 384	-	linker

J: 385 - 411 (UniProt: P02976)

PDB	External Database	Details
Glu 412	-	linker
His 413	-	linker
Gln 414	-	linker
Ala 415	-	linker
Pro 416	-	linker
Lys 417	-	linker
Gly 418	-	linker
Gly 419	-	linker
Ser 420	-	linker
Gly 421	-	linker
Gly 422	-	linker
Ala 423	-	linker
Gly 424	-	linker
Ser 425	-	linker
Gly 426	-	linker

J: 427 - 475 (UniProt: P99134)

PDB	External Database	Details
Gly 476	-	linker
Gly 477	-	linker
Gly 478	-	linker
Ser 479	-	linker
Gly 480	-	linker
Gly 481	-	linker
Gly 482	-	linker
Ser 483	-	linker
Gly 484	-	linker
Gly 485	-	linker
Ser 486	-	linker

J: 487 - 544 (UniProt: P19909)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	247121.1
Branched	Number of molecules	2
	Total formula weight	684.6
All*	Total formula weight	247805.7

*Water molecules are not included.