9Q9I
Cryo-EM structure of human Mre11-Rad50-Nbs1 complex bound to DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (P) | DNA (50-MER) | polymer | 50 | 15164.7 | 1 | Homo sapiens | ||
| 2 | B (T) | DNA (50-MER) | polymer | 50 | 15615.4 | 1 | Homo sapiens | ||
| 3 | C (F) | Nibrin | polymer | 754 | 85073.0 | 1 | UniProt (O60934) Pfam (PF00498) Pfam (PF00533) Pfam (PF16508) Pfam (PF08599) | Homo sapiens (human) | Cell cycle regulatory protein p95,Nijmegen breakage syndrome protein 1 |
| 4 | D, E (A, B) | DNA repair protein RAD50 | polymer | 1312 | 154150.0 | 2 | UniProt (Q92878) Pfam (PF13476) Pfam (PF04423) Pfam (PF13558) | Homo sapiens (human) | |
| 5 | F, G (D, E) | Double-strand break repair protein MRE11 | polymer | 738 | 84008.6 | 2 | UniProt (P49959) Pfam (PF00149) Pfam (PF04152) | Homo sapiens (human) | Double-strand break repair protein MRE11A,Meiotic recombination 11 homolog 1,MRE11 homolog 1,Meiotic recombination 11 homolog A,MRE11 homolog A |
| 6 | H, L (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | I, M (A, B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 2 | Chemie (ADP) | |||
| 8 | J, K (A) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 2 | Chemie (BEF) | |||
| 9 | N, O (D, E) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 10 | P, Q (A, B) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
D, E: 1 - 708 (UniProt: P49959)
| PDB | External Database | Details |
|---|---|---|
| Asn 129 | His 129 | engineered mutation |
| Ser 709 | - | expression tag |
| Gly 710 | - | expression tag |
| Gly 711 | - | expression tag |
| Ser 712 | - | expression tag |
| Leu 713 | - | expression tag |
| Glu 714 | - | expression tag |
| Val 715 | - | expression tag |
| Leu 716 | - | expression tag |
| Phe 717 | - | expression tag |
| Gln 718 | - | expression tag |
| Gly 719 | - | expression tag |
| Pro 720 | - | expression tag |
| Asp 721 | - | expression tag |
| Tyr 722 | - | expression tag |
| Lys 723 | - | expression tag |
| Asp 724 | - | expression tag |
| Asp 725 | - | expression tag |
| Asp 726 | - | expression tag |
| Asp 727 | - | expression tag |
| Lys 728 | - | expression tag |
| Gly 729 | - | expression tag |
| Thr 730 | - | expression tag |
| Asp 731 | - | expression tag |
| Tyr 732 | - | expression tag |
| Lys 733 | - | expression tag |
| Asp 734 | - | expression tag |
| Asp 735 | - | expression tag |
| Asp 736 | - | expression tag |
| Asp 737 | - | expression tag |
| Lys 738 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 7 |
| Total formula weight | 592170.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1144.9 | |
| All* | Total formula weight | 593315.3 |
