9Q96
Cryo-EM Structure of Bacterial RNA polymerase-sigma54 transcription open complex with wild type sigma54, from RPi(-10-1)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F (M) | RNA polymerase sigma-54 factor | polymer | 497 | 56150.0 | 1 | UniProt (A0A377VEN9) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) | Klebsiella pneumoniae | |
| 6 | G (N) | NIFH PROMOTER NON-TEMPLATE DNA | polymer | 46 | 14215.1 | 1 | Klebsiella pneumoniae | ||
| 7 | H (T) | NIFH PROMOTER TEMPLATE DNA | polymer | 46 | 14172.1 | 1 | Klebsiella pneumoniae |
Sequence modifications
M: 26 - 477 (UniProt: A0A377VEN9)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Met 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Gln 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Arg 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Ser 10 | - | expression tag |
| Gln 11 | - | expression tag |
| Gln 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Ala 14 | - | expression tag |
| Met 15 | - | expression tag |
| Thr 16 | - | expression tag |
| Pro 17 | - | expression tag |
| Gln 18 | - | expression tag |
| Leu 19 | - | expression tag |
| Gln 20 | - | expression tag |
| Gln 21 | - | expression tag |
| Ala 22 | - | expression tag |
| Ile 23 | - | expression tag |
| Arg 24 | - | expression tag |
| Leu 25 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 474091.7 | |
| All* | Total formula weight | 474091.7 |
