9Q90
CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (1, 2, 3, 4, 5...) | Psp operon transcriptional activator | polymer | 275 | 31016.4 | 6 | UniProt (P37344) Pfam (PF00158) | Escherichia coli K-12 | Phage shock protein F |
| 2 | G, H (A, B) | DNA-directed RNA polymerase subunit alpha | polymer | 329 | 36558.7 | 2 | UniProt (P0A7Z4) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) | Escherichia coli K-12 | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 3 | I (C) | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 1 | UniProt (P0A8V2) Pfam (PF00562) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) | Escherichia coli K-12 | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 4 | J (D) | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 1 | UniProt (P0A8T7) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) | Escherichia coli K-12 | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 5 | K (E) | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 1 | UniProt (P0A800) Pfam (PF01192) | Escherichia coli K-12 | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 6 | L (M) | RNA polymerase sigma-54 factor | polymer | 497 | 56150.0 | 1 | UniProt (A6TEM1) Pfam (PF00309) Pfam (PF04963) Pfam (PF04552) UniProt (by SIFTS) (B5XST9) | Klebsiella pneumoniae | |
| 7 | M (N) | DNA (36-MER) | polymer | 36 | 11079.1 | 1 | Escherichia coli K-12 | ||
| 8 | N (T) | DNA (36-MER) | polymer | 36 | 11079.1 | 1 | Escherichia coli K-12 | ||
| 9 | AA, O, R, U, X (6, 1, 2, 3, 4) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 5 | Chemie (ADP) | |||
| 10 | P, S, V, Y (1, 2, 3, 4) | ALUMINUM FLUORIDE | non-polymer | 84.0 | 4 | Chemie (AF3) | |||
| 11 | Q, T, W, Z (1, 2, 3, 4) | MAGNESIUM ION | non-polymer | 24.3 | 4 | Chemie (MG) |
Sequence modifications
M: 15 - 477 (UniProt: A6TEM1)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | initiating methionine |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Met 1 | - | expression tag |
| Lys 2 | - | expression tag |
| Gln 3 | - | expression tag |
| Gly 4 | - | expression tag |
| Leu 5 | - | expression tag |
| Gln 6 | - | expression tag |
| Leu 7 | - | expression tag |
| Arg 8 | - | expression tag |
| Leu 9 | - | expression tag |
| Ser 10 | - | expression tag |
| Gln 11 | - | expression tag |
| Gln 12 | - | expression tag |
| Leu 13 | - | expression tag |
| Ala 14 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 653961.4 | |
| Non-Polymers* | Number of molecules | 13 |
| Total formula weight | 2569.1 | |
| All* | Total formula weight | 656530.5 |
