9Q8Y

Structure of RNF38 RING with linchpin mutant R454Y in complex with Ubch5B-Ub

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Ubiquitin-conjugating enzyme E2 D2	polymer	146	16720.2	2	UniProt (P62837) Pfam (PF00179)	Homo sapiens (human)	(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1
2	C, D (C, D)	Isoform 2 of E3 ubiquitin-protein ligase RNF38	polymer	69	8041.2	2	UniProt (Q9H0F5) Pfam (PF13639)	Homo sapiens (human)	RING finger protein 38,RING-type E3 ubiquitin transferase RNF38
3	E, F (F, G)	Ubiquitin-40S ribosomal protein S27a	polymer	77	8663.9	2	UniProt (J3QTR3) Pfam (PF00240) UniProt (by SIFTS) (P0CG48)	Homo sapiens (human)
4	G, H, I, J (C, D)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5	K, L (B, D)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, B: 2 - 147 (UniProt: P62837)

PDB	External Database	Details
Arg 22	Ser 22	engineered mutation
Lys 85	Cys 85	engineered mutation

C, D: 390 - 458 (UniProt: Q9H0F5)

PDB	External Database	Details
Tyr 454	Arg 454	engineered mutation

F, G: 1 - 76 (UniProt: J3QTR3)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	66850.6
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	67112.2

*Water molecules are not included.