9Q2L

Rad55-Rad57(E161Q)-SHU-3xRad51 bound to ssDNA with ATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Methylated-DNA--protein-cysteine methyltransferase,DNA repair protein RAD55	polymer	631	70243.6	1	UniProt (E5BBQ0) UniProt (P38953) Pfam (PF06745)	Saccharomyces cerevisiae (brewer's yeast)	6-O-methylguanine-DNA methyltransferase,O-6-methylguanine-DNA-alkyltransferase
2	B (B)	DNA repair protein RAD57	polymer	460	52307.3	1	UniProt (P25301) Pfam (PF08423)	Saccharomyces cerevisiae (brewer's yeast)
3	C (C)	Chromosome segregation in meiosis protein 2	polymer	213	24983.7	1	UniProt (P40465) Pfam (PF16834)	Saccharomyces cerevisiae (brewer's yeast)
4	D (D)	Platinum sensitivity protein 3	polymer	281	32385.1	1	UniProt (Q12318) Pfam (PF16836)	Saccharomyces cerevisiae (brewer's yeast)
5	E (E)	Suppressor of HU sensitivity involved in recombination protein 1	polymer	150	17138.6	1	UniProt (P38751)	Saccharomyces cerevisiae (brewer's yeast)
6	F (F)	Suppressor of hydroxyurea sensitivity protein 2	polymer	262	30179.9	1	UniProt (C7GVQ9)	Saccharomyces cerevisiae (brewer's yeast)
7	G, I, J (G, I, J)	DNA repair protein RAD51	polymer	418	45239.7	3	UniProt (P25454) Pfam (PF14520) Pfam (PF08423)	Saccharomyces cerevisiae (brewer's yeast)
8	H (H)	ssDNA (13-mer)	polymer	13	3909.5	1		synthetic construct
9	K (A)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
10	L, N, Q, R (A, B, G, I)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
11	M, P (B, G)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
12	O (F)	ZINC ION	non-polymer		65.4	1	Chemie (ZN) PubChem (32051)

Sequence modifications

A: -190 - -14 (UniProt: E5BBQ0)

PDB	External Database	Details
Met -224	-	expression tag
Ser -223	-	expression tag
Ala -222	-	expression tag
Trp -221	-	expression tag
Ser -220	-	expression tag
His -219	-	expression tag
Pro -218	-	expression tag
Gln -217	-	expression tag
Phe -216	-	expression tag
Glu -215	-	expression tag
Lys -214	-	expression tag
Gly -213	-	expression tag
Gly -212	-	expression tag
Gly -211	-	expression tag
Ser -210	-	expression tag
Gly -209	-	expression tag
Gly -208	-	expression tag
Gly -207	-	expression tag
Ser -206	-	expression tag
Gly -205	-	expression tag
Gly -204	-	expression tag
Ser -203	-	expression tag
Ala -202	-	expression tag
Trp -201	-	expression tag
Ser -200	-	expression tag
His -199	-	expression tag
Pro -198	-	expression tag
Gln -197	-	expression tag
Phe -196	-	expression tag
Glu -195	-	expression tag
Lys -194	-	expression tag
Gly -193	-	expression tag
Ser -192	-	expression tag
Met -191	-	expression tag
Arg -162	Glu 33	conflict
Pro -13	-	linker
Gly -12	-	linker
Leu -11	-	linker
Gly -10	-	linker
Gly -9	-	linker
Ser -8	-	linker
Glu -7	-	linker
Asn -6	-	linker
Leu -5	-	linker
Tyr -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Ser 0	-	linker

A: 1 - 406 (UniProt: P38953)
B: 1 - 460 (UniProt: P25301)

PDB	External Database	Details
Gln 161	Glu 161	engineered mutation

D: 1 - 242 (UniProt: Q12318)

PDB	External Database	Details
Met -38	-	initiating methionine
Ser -37	-	expression tag
Ala -36	-	expression tag
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag

F: 2 - 223 (UniProt: C7GVQ9)

PDB	External Database	Details
Met -38	-	initiating methionine
Ser -37	-	expression tag
Ala -36	-	expression tag
Trp -35	-	expression tag
Ser -34	-	expression tag
His -33	-	expression tag
Pro -32	-	expression tag
Gln -31	-	expression tag
Phe -30	-	expression tag
Glu -29	-	expression tag
Lys -28	-	expression tag
Gly -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Gly -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Trp -15	-	expression tag
Ser -14	-	expression tag
His -13	-	expression tag
Pro -12	-	expression tag
Gln -11	-	expression tag
Phe -10	-	expression tag
Glu -9	-	expression tag
Lys -8	-	expression tag
Ser -7	-	expression tag
Gly -6	-	expression tag
Glu -5	-	expression tag
Asn -4	-	expression tag
Leu -3	-	expression tag
Tyr -2	-	expression tag
Phe -1	-	expression tag
Gln 0	-	expression tag
Gly 1	-	expression tag

G, I, J: 1 - 400 (UniProt: P25454)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
Gly -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	366867.0
Non-Polymers*	Number of molecules	8
	Total formula weight	1604.2
All*	Total formula weight	368471.2

*Water molecules are not included.