9Q0W

Cryo-EM Structure of HIV-1 BG505DS-SOSIP.664 Env Trimer Bound to DFPH-a.01_10R59P_LC Fab

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E (A, C, E)	HIV-1 BG505 DS-SOSIP gp120	polymer	481	54086.3	3	UniProt (Q2N0S6)	Human immunodeficiency virus 1
2	B, D, F (B, D, F)	BG505 DS-SOSIP GP41	polymer	153	17146.5	3	UniProt (Q2N0S5)	Human immunodeficiency virus 1
3	G, I, K (G, I, K)	DFPH-a.01_10R59P_LC Fab heavy chain	polymer	233	24875.8	3		Macaca
4	H, J, L (H, J, L)	DFPH-a.01_10R59P_LC Fab light chain	polymer	214	23266.8	3		Macaca
5	AA, BA, DA, EA, FA... (Y, Z, d, e, f...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	27	In PDB GlyTouCan (G42666HT)
6	CA, MA, S (b, m, R)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G42227JK)
7	AB, BB, CB, DB, EB... (C, E, A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	18	Chemie (NAG)

Sequence modifications

A, C, E: 31 - 508 (UniProt: Q2N0S6)

PDB	External Database	Details
Cys 201	Ile 200	conflict
Asn 332	Thr 330	conflict
Cys 433	Ala 430	conflict
Cys 501	Ala 498	conflict
Arg 509	-	expression tag
Arg 510	-	expression tag
Arg 511	-	expression tag
Arg 512	-	expression tag
Arg 513	-	expression tag

B, D, F: 512 - 664 (UniProt: Q2N0S5)

PDB	External Database	Details
Pro 559	Ile 556	engineered mutation
Cys 605	Thr 602	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	358126.0
Branched	Number of molecules	30
	Total formula weight	14191.3
Non-Polymers*	Number of molecules	18
	Total formula weight	3981.7
All*	Total formula weight	376299.1

*Water molecules are not included.