9Q0O

Cryo-EM structure of PPAT-NUDT5 complex bound to 6-benzylthioinosine-5'-monophosphate (6-benzylTIMP)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Amidophosphoribosyltransferase	polymer	552	60690.3	4	UniProt (Q06203)	Homo sapiens (human)	ATase,Glutamine phosphoribosylpyrophosphate amidotransferase,GPAT
2	E, F, G, H (E, F, G, H)	ADP-sugar pyrophosphatase	polymer	223	24523.7	4	UniProt (Q9UKK9)	Homo sapiens (human)	8-oxo-dGDP phosphatase,Nuclear ATP-synthesis protein NUDIX5,Nucleoside diphosphate-linked moiety X motif 5,Nudix motif 5,hNUDT5,YSA1H
3	I, K, M, O (A, B, C, D)	IRON/SULFUR CLUSTER	non-polymer		351.6	4	Chemie (SF4)
4	J, L, N, P (A, B, C, D)	6-(benzylsulfanyl)-9-(5-O-phosphono-beta-D-ribofuranosyl)-9H-purine	non-polymer		454.4	4	Chemie (A1CQ4)

Sequence modifications

A, B, C, D: 12 - 517 (UniProt: Q06203)

PDB	External Database	Details
Gly 518	-	expression tag
Gly 519	-	expression tag
Ser 520	-	expression tag
Lys 521	-	expression tag
Pro 522	-	expression tag
Ile 523	-	expression tag
Pro 524	-	expression tag
Asn 525	-	expression tag
Pro 526	-	expression tag
Leu 527	-	expression tag
Leu 528	-	expression tag
Gly 529	-	expression tag
Leu 530	-	expression tag
Asp 531	-	expression tag
Ser 532	-	expression tag
Thr 533	-	expression tag
Ser 534	-	expression tag
Ala 535	-	expression tag
Trp 536	-	expression tag
Ser 537	-	expression tag
His 538	-	expression tag
Pro 539	-	expression tag
Gln 540	-	expression tag
Phe 541	-	expression tag
Glu 542	-	expression tag
Lys 543	-	expression tag
Gly 544	-	expression tag
Gly 545	-	expression tag
Gly 546	-	expression tag
Ser 547	-	expression tag
Gly 548	-	expression tag
Gly 549	-	expression tag
Gly 550	-	expression tag
Ser 551	-	expression tag
Gly 552	-	expression tag
Gly 553	-	expression tag
Gly 554	-	expression tag
Ala 555	-	expression tag
Trp 556	-	expression tag
Ser 557	-	expression tag
His 558	-	expression tag
Pro 559	-	expression tag
Gln 560	-	expression tag
Phe 561	-	expression tag
Glu 562	-	expression tag
Lys 563	-	expression tag

E, F, G, H: 2 - 219 (UniProt: Q9UKK9)

PDB	External Database	Details
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Phe 74	Tyr 74	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	340855.9
Non-Polymers*	Number of molecules	8
	Total formula weight	3224.1
All*	Total formula weight	344080.0

*Water molecules are not included.