9PXU

Inactive-state naloxone-mu opioid receptor nanobody6 complex

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,Mu-type opioid receptor	polymer	622	69550.3	1	UniProt (P0ABE7) UniProt (P35372) Pfam (PF07361) Pfam (PF00001)	Homo sapiens (human)	Cytochrome b-562,M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
2	B (C)	Nanobody6	polymer	131	14418.9	1		synthetic construct
3	C (K)	Anti-fab nanobody	polymer	126	13764.1	1		synthetic construct
4	D (L)	Nab-fab light chain	polymer	215	23258.8	1		synthetic construct
5	E (H)	Nab-fab heavy chain	polymer	239	25684.5	1		synthetic construct
6	F (R)	Naloxone	non-polymer		327.4	1	Chemie (A1APV)
7	G (R)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)

Sequence modifications

R: -108 - -8 (UniProt: P0ABE7)

PDB	External Database	Details
Met -169	-	expression tag
Lys -168	-	expression tag
Thr -167	-	expression tag
Ile -166	-	expression tag
Ile -165	-	expression tag
Ala -164	-	expression tag
Leu -163	-	expression tag
Ser -162	-	expression tag
Tyr -161	-	expression tag
Ile -160	-	expression tag
Phe -159	-	expression tag
Cys -158	-	expression tag
Leu -157	-	expression tag
Val -156	-	expression tag
Phe -155	-	expression tag
Ala -154	-	expression tag
Asp -153	-	expression tag
Tyr -152	-	expression tag
Lys -151	-	expression tag
Asp -150	-	expression tag
His -149	-	expression tag
Asp -148	-	expression tag
Gly -147	-	expression tag
Asp -146	-	expression tag
Tyr -145	-	expression tag
Lys -144	-	expression tag
Asp -143	-	expression tag
His -142	-	expression tag
Asp -141	-	expression tag
Ile -140	-	expression tag
Asp -139	-	expression tag
Tyr -138	-	expression tag
Lys -137	-	expression tag
Asp -136	-	expression tag
Asp -135	-	expression tag
Asp -134	-	expression tag
Asp -133	-	expression tag
Lys -132	-	expression tag
Leu -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
His -127	-	expression tag
His -126	-	expression tag
His -125	-	expression tag
His -124	-	expression tag
His -123	-	expression tag
His -122	-	expression tag
His -121	-	expression tag
Leu -120	-	expression tag
Glu -119	-	expression tag
Val -118	-	expression tag
Leu -117	-	expression tag
Phe -116	-	expression tag
Gln -115	-	expression tag
Gly -114	-	expression tag
Pro -113	-	expression tag
Gly -112	-	expression tag
Ser -111	-	expression tag
Gly -110	-	expression tag
Ser -109	-	expression tag
Trp -102	Met 29	conflict
Ile -7	-	linker
Gln -6	-	linker
Lys -5	-	linker
Tyr -4	-	linker
Leu -3	-	linker
Gly -2	-	linker
Thr -1	-	linker
Gly 0	-	linker
Ser 1	-	linker

R: 2 - 400 (UniProt: P35372)

PDB	External Database	Details
Leu 266	Met 266	conflict
Arg 271	Lys 271	conflict
Ala 401	-	expression tag
Ala 402	-	expression tag
Ala 403	-	expression tag
Leu 404	-	expression tag
Glu 405	-	expression tag
Val 406	-	expression tag
Leu 407	-	expression tag
Phe 408	-	expression tag
Gln 409	-	expression tag
Gly 410	-	expression tag
Pro 411	-	expression tag
Gly 412	-	expression tag
Ser 413	-	expression tag
Trp 414	-	expression tag
Ser 415	-	expression tag
His 416	-	expression tag
Pro 417	-	expression tag
Gln 418	-	expression tag
Phe 419	-	expression tag
Glu 420	-	expression tag
Lys 421	-	expression tag
Gly 422	-	expression tag
Gly 423	-	expression tag
Gly 424	-	expression tag
Ser 425	-	expression tag
Gly 426	-	expression tag
Gly 427	-	expression tag
Gly 428	-	expression tag
Ser 429	-	expression tag
Gly 430	-	expression tag
Gly 431	-	expression tag
Ser 432	-	expression tag
Ser 433	-	expression tag
Ala 434	-	expression tag
Trp 435	-	expression tag
Ser 436	-	expression tag
His 437	-	expression tag
Pro 438	-	expression tag
Gln 439	-	expression tag
Phe 440	-	expression tag
Glu 441	-	expression tag
Lys 442	-	expression tag
His 443	-	expression tag
His 444	-	expression tag
His 445	-	expression tag
His 446	-	expression tag
His 447	-	expression tag
His 448	-	expression tag
His 449	-	expression tag
His 450	-	expression tag
His 451	-	expression tag
His 452	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	146676.6
Non-Polymers*	Number of molecules	2
	Total formula weight	350.4
All*	Total formula weight	147027.0

*Water molecules are not included.