9PUD

Cryo-EM structure of MOR-Gi1 complex protein bound with DAMGO

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, F (A, C)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	2	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B, G (B, D)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	342	37561.1	2	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C, H (G, E)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	80	8506.8	2	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D, I (R, F)	Mu-type opioid receptor	polymer	388	43577.8	2	UniProt (P35372)	Homo sapiens (human)	M-OR-1,MOR-1,Mu opiate receptor,Mu opioid receptor,MOP,hMOP
5	E, J (S, H)	DAMGO	polymer	5	513.6	2	BIRD (PRD_002308)	Homo sapiens
6	K, L, M, N (R, F)	CHOLESTEROL	non-polymer		386.7	4	Chemie (CLR) PubChem (5997)

Sequence modifications

A, C: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

B, D: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -1	-	initiating methionine
Ser 0	-	expression tag
Gly 1	-	expression tag

G, E: 1 - 71 (UniProt: P59768)

PDB	External Database	Details
Gly 72	-	expression tag
Ser 73	-	expression tag
Ala 74	-	expression tag
Gly 75	-	expression tag
Ser 76	-	expression tag
Ala 77	-	expression tag
Gly 78	-	expression tag
Ser 79	-	expression tag
Ala 80	-	expression tag

R, F: 1 - 388 (UniProt: P35372)

PDB	External Database	Details
Trp 158	Phe 158	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	261146.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1546.6
All*	Total formula weight	262693.2

*Water molecules are not included.