9PR2
Crystal structure of thermostable variant of phosphite dehydrogenase from Pseudomonas stutzeri in complex with NAD
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phosphonate dehydrogenase | polymer | 339 | 36852.3 | 1 | UniProt (O69054) | Stutzerimonas stutzeri | NAD-dependent phosphite dehydrogenase |
| 2 | B (A) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 1 | Chemie (NAD) | |||
| 3 | C, D (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) PubChem (312) | |||
| 4 | E, F (A) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 5 | G (A) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) PubChem (175) | |||
| 6 | H (A) | water | water | 18.0 | 199 | Chemie (HOH) |
Sequence modifications
A: 1 - 336 (UniProt: O69054)
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| Glu 13 | Asp 13 | engineered mutation |
| Ile 26 | Met 26 | engineered mutation |
| Ile 71 | Val 71 | engineered mutation |
| Lys 130 | Glu 130 | engineered mutation |
| Arg 132 | Gln 132 | engineered mutation |
| Arg 137 | Gln 137 | engineered mutation |
| Phe 150 | Ile 150 | engineered mutation |
| Leu 215 | Gln 215 | engineered mutation |
| Gln 275 | Arg 275 | engineered mutation |
| Gln 276 | Leu 276 | engineered mutation |
| Leu 313 | Ile 313 | engineered mutation |
| Ala 315 | Val 315 | engineered mutation |
| Glu 319 | Ala 319 | engineered mutation |
| Val 325 | Ala 325 | engineered mutation |
| Asn 332 | Glu 332 | engineered mutation |
| Asp 336 | Cys 336 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 36852.3 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 977.6 | |
| All* | Total formula weight | 37829.9 |
