9PJD

Cryo-EM structure of the ClpXP AAA+ protease bound to an unidentified portion of lambdaO-tagged Arc substrate within a translocation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	424	46414.8	6	UniProt (C3TLS7)	Escherichia coli
2	G (G)	unidentified portion of the substrate	polymer	6	465.7	1		Escherichia coli
3	H, I, J, K, L... (H, I, J, K, L...)	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	14	UniProt (P0A6G7) Pfam (PF00574)	Escherichia coli	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
4	AA, CA, EA, V, W... (D, E, F, A, B...)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	6	Chemie (AGS)
5	BA, DA, FA, X, Z (D, E, F, B, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG) PubChem (888)

Sequence modifications

A, B, C, D, E, F: 1 - 424 (UniProt: C3TLS7)

PDB	External Database	Details
Glu 408	Lys 408	conflict

H, I, J, K, L, M, N, O, P, Q, R, S, T, U: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	607518.9
Non-Polymers*	Number of molecules	11
	Total formula weight	3261.0
All*	Total formula weight	610779.9

*Water molecules are not included.