9PIO

Cryo-EM structure of the ClpXP AAA+ protease bound to lambdaO-tagged Arc in a recognition complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	ATP-dependent Clp protease ATP-binding subunit ClpX	polymer	424	46414.8	6	UniProt (C3TLS7) Pfam (PF06689) Pfam (PF07724) Pfam (PF10431)	Escherichia coli
2	G (G)	Arc family DNA-binding protein	polymer	79	9256.6	1	UniProt (A0A1Y5CGZ0) Pfam (PF03869)	Escherichia coli	Putative transcriptional repressor
3	H, I, J, K, L... (H, I, J, K, L...)	ATP-dependent Clp protease proteolytic subunit	polymer	207	23468.9	14	UniProt (P0A6G7) Pfam (PF00574)	Escherichia coli	Caseinolytic protease,Endopeptidase Clp,Heat shock protein F21.5,Protease Ti
4	BA, DA, V, X, Z (E, F, B, C, D)	PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER	non-polymer		523.2	5	Chemie (AGS)
5	AA, CA, W, Y (D, E, B)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)

Sequence modifications

A, B, C, D, E, F: 1 - 424 (UniProt: C3TLS7)

PDB	External Database	Details
Glu 408	Lys 408	conflict

G: 17 - 68 (UniProt: A0A1Y5CGZ0)

PDB	External Database	Details
Met 1	-	initiating methionine
Thr 2	-	expression tag
Asn 3	-	expression tag
Thr 4	-	expression tag
Ala 5	-	expression tag
Lys 6	-	expression tag
Ile 7	-	expression tag
Leu 8	-	expression tag
Asn 9	-	expression tag
Phe 10	-	expression tag
Gly 11	-	expression tag
Arg 12	-	expression tag
Ala 13	-	expression tag
Ser 14	-	expression tag
Met 15	-	expression tag
Gly 16	-	expression tag
Arg 31	Lys 16	conflict
His 69	-	expression tag
His 70	-	expression tag
His 71	-	expression tag
His 72	-	expression tag
His 73	-	expression tag
His 74	-	expression tag
Lys 75	-	expression tag
Asn 76	-	expression tag
Gln 77	-	expression tag
His 78	-	expression tag
Asp 79	-	expression tag

H, I, J, K, L, M, N, O, P, Q, R, S, T, U: 2 - 193 (UniProt: P0A6G7)

PDB	External Database	Details
Glu 194	-	expression tag
Asn 195	-	expression tag
Leu 196	-	expression tag
Tyr 197	-	expression tag
Phe 198	-	expression tag
Gln 199	-	expression tag
Ser 200	-	expression tag
Leu 201	-	expression tag
Glu 202	-	expression tag
His 203	-	expression tag
His 204	-	expression tag
His 205	-	expression tag
His 206	-	expression tag
His 207	-	expression tag
His 208	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	21
	Total formula weight	616309.9
Non-Polymers*	Number of molecules	9
	Total formula weight	2713.5
All*	Total formula weight	619023.3

*Water molecules are not included.