9PIG
Cryo-EM structure of human NCC with indapamide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, B) | Solute carrier family 12 member 3 | polymer | 890 | 98893.1 | 2 | UniProt (P55017) | Homo sapiens (human) | Na-Cl cotransporter,NCC,Na-Cl symporter,Thiazide-sensitive sodium-chloride cotransporter |
| 2 | B, D (D, C) | Solute carrier family 12 member 2 | polymer | 202 | 21497.1 | 2 | UniProt (A0A0G2KTI4) | Danio rerio (zebrafish) | Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2,BSC2,Na-K-Cl cotransporter 1,NKCC1 |
| 3 | E, I (A, B) | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) PubChem (923) | |||
| 4 | F, J (A, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 2 | Chemie (NAG) PubChem (24139) | |||
| 5 | G, K (A, B) | 4-chloro-N-[(2S)-2-methyl-2,3-dihydro-1H-indol-1-yl]-3-sulfamoylbenzamide | non-polymer | 365.8 | 2 | Chemie (BL1) PubChem (12598931) | |||
| 6 | H, L (A, B) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 2 | Chemie (ATP) | |||
| 7 | M, N (A, B) | water | water | 18.0 | 2 | Chemie (HOH) |
Sequence modifications
A, B: 132 - 1021 (UniProt: P55017)
D, C: 1 - 202 (UniProt: A0A0G2KTI4)
| PDB | External Database | Details |
|---|---|---|
| Ala 240 | Glu 240 | engineered mutation |
| Gly 264 | Ala 264 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Lys 76 | Glu 76 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 240780.6 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 2234.4 | |
| All* | Total formula weight | 243015.0 |
