9PF1

Nub1/Fat10-processing human 26S proteasome with Rpt4 at top of spiral staircase (AAA+ motor locally refined)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	26S proteasome regulatory subunit 7	polymer	433	48700.8	1	UniProt (P35998)	Homo sapiens (human)	26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2,Protein MSS1
2	B (C)	26S protease regulatory subunit 8	polymer	406	45694.0	1	UniProt (P62195)	Homo sapiens (human)	26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG
3	C (G)	Proteasome subunit alpha type-6	polymer	246	27432.5	1	UniProt (P60900)	Homo sapiens (human)	27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain
4	D (H)	Proteasome subunit alpha type-2	polymer	234	25934.5	1	UniProt (P25787)	Homo sapiens (human)	Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3,Proteasome subunit alpha-2,alpha-2
5	E (I)	Proteasome subunit alpha type-4	polymer	261	29525.8	1	UniProt (P25789)	Homo sapiens (human)	Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L
6	F (J)	Proteasome subunit alpha type-7	polymer	248	27929.9	1	UniProt (O14818)	Homo sapiens (human)	Proteasome subunit RC6-1,Proteasome subunit XAPC7,Proteasome subunit alpha-4,alpha-4
7	G (L)	Proteasome subunit alpha type-1	polymer	263	29595.6	1	UniProt (P25786)	Homo sapiens (human)	30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain
8	H (M)	Proteasome subunit alpha type-3	polymer	255	28469.3	1	UniProt (P25788)	Homo sapiens (human)	Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8
9	I (c)	26S proteasome non-ATPase regulatory subunit 14	polymer	424	46940.9	1	UniProt (O00487)	Homo sapiens (human)
10	J (v)	Substrate polypeptide	polymer	12	1039.3	1		Homo sapiens (human)
11	K (B)	26S proteasome regulatory subunit 4	polymer	440	49260.5	1	UniProt (P62191)	Homo sapiens (human)	P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1
12	L (D)	26S proteasome regulatory subunit 6B	polymer	418	47368.1	1	UniProt (P43686)	Homo sapiens (human)	26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7
13	M (E)	26S protease regulatory subunit 10B	polymer	389	44241.0	1	UniProt (P62333)	Homo sapiens (human)	26S proteasome AAA-ATPase subunit RPT4,Proteasome 26S subunit ATPase 6,Proteasome subunit p42
14	N (F)	26S proteasome regulatory subunit 6A	polymer	439	49266.5	1	UniProt (P17980)	Homo sapiens (human)	26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1
15	O (K)	Proteasome subunit alpha type-5	polymer	241	26436.0	1	UniProt (P28066)	Homo sapiens (human)	Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome subunit alpha-5,alpha-5,Proteasome zeta chain
16	P, Q, S, T (A, C, B, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
17	R (c)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
18	U, W (E, F)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
19	V, X (E, F)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

H: 1 - 234 (UniProt: P25787)

PDB	External Database	Details
His 163	Met 163	conflict

c: 1 - 310 (UniProt: O00487)

PDB	External Database	Details
Leu 311	-	expression tag
Ile 312	-	expression tag
Asn 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag
His 319	-	expression tag
Asp 320	-	expression tag
Tyr 321	-	expression tag
Asp 322	-	expression tag
Ile 323	-	expression tag
Pro 324	-	expression tag
Thr 325	-	expression tag
Thr 326	-	expression tag
Ala 327	-	expression tag
Ser 328	-	expression tag
Glu 329	-	expression tag
Asn 330	-	expression tag
Leu 331	-	expression tag
Tyr 332	-	expression tag
Phe 333	-	expression tag
Gln 334	-	expression tag
Gly 335	-	expression tag
Glu 336	-	expression tag
Leu 337	-	expression tag
Gly 338	-	expression tag
Met 339	-	expression tag
Arg 340	-	expression tag
Gly 341	-	expression tag
Ser 342	-	expression tag
Ala 343	-	expression tag
Gly 344	-	expression tag
Lys 345	-	expression tag
Ala 346	-	expression tag
Gly 347	-	expression tag
Glu 348	-	expression tag
Gly 349	-	expression tag
Glu 350	-	expression tag
Ile 351	-	expression tag
Pro 352	-	expression tag
Ala 353	-	expression tag
Pro 354	-	expression tag
Leu 355	-	expression tag
Ala 356	-	expression tag
Gly 357	-	expression tag
Thr 358	-	expression tag
Val 359	-	expression tag
Ser 360	-	expression tag
Lys 361	-	expression tag
Ile 362	-	expression tag
Leu 363	-	expression tag
Val 364	-	expression tag
Lys 365	-	expression tag
Glu 366	-	expression tag
Gly 367	-	expression tag
Asp 368	-	expression tag
Thr 369	-	expression tag
Val 370	-	expression tag
Lys 371	-	expression tag
Ala 372	-	expression tag
Gly 373	-	expression tag
Gln 374	-	expression tag
Thr 375	-	expression tag
Val 376	-	expression tag
Leu 377	-	expression tag
Val 378	-	expression tag
Leu 379	-	expression tag
Glu 380	-	expression tag
Ala 381	-	expression tag
Met 382	-	expression tag
Lys 383	-	expression tag
Met 384	-	expression tag
Glu 385	-	expression tag
Thr 386	-	expression tag
Glu 387	-	expression tag
Ile 388	-	expression tag
Asn 389	-	expression tag
Ala 390	-	expression tag
Pro 391	-	expression tag
Thr 392	-	expression tag
Asp 393	-	expression tag
Gly 394	-	expression tag
Lys 395	-	expression tag
Val 396	-	expression tag
Glu 397	-	expression tag
Lys 398	-	expression tag
Val 399	-	expression tag
Leu 400	-	expression tag
Val 401	-	expression tag
Lys 402	-	expression tag
Glu 403	-	expression tag
Arg 404	-	expression tag
Asp 405	-	expression tag
Ala 406	-	expression tag
Val 407	-	expression tag
Gln 408	-	expression tag
Gly 409	-	expression tag
Gly 410	-	expression tag
Gln 411	-	expression tag
Gly 412	-	expression tag
Leu 413	-	expression tag
Ile 414	-	expression tag
Lys 415	-	expression tag
Ile 416	-	expression tag
Gly 417	-	expression tag
Val 418	-	expression tag
His 419	-	expression tag
His 420	-	expression tag
His 421	-	expression tag
His 422	-	expression tag
His 423	-	expression tag
His 424	-	expression tag

D: 1 - 418 (UniProt: P43686)

PDB	External Database	Details
Ala 146	Glu 146	conflict

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	527834.6
Non-Polymers*	Number of molecules	9
	Total formula weight	2837.2
All*	Total formula weight	530671.8

*Water molecules are not included.