9PD8

22bin20S complex (NSF-alphaSNAP-2:2 syntaxin-1a:SNAP-25), hydrolyzing, class 21

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Vesicle-fusing ATPase	polymer	747	82883.4	6	UniProt (P18708) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) Pfam (PF21964)	Cricetulus griseus (Chinese hamster)	N-ethylmaleimide-sensitive fusion protein,NEM-sensitive fusion protein,Vesicular-fusion protein NSF
2	G, J (J, I)	Synaptosomal-associated protein 25,Synaptosomal-associated protein 25,Synaptosomal-associated protein 25,Alpha-soluble NSF attachment protein	polymer	518	58309.3	2	UniProt (P60881) Pfam (PF00835)	Rattus norvegicus (Norway rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
3	H, I (G, H)	Syntaxin-1A	polymer	267	31045.9	2	UniProt (P32851) Pfam (PF00804) Pfam (PF05739)	Rattus norvegicus (Norway rat)	Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A
4	K, L, M, N (K, L, M, N)	Alpha-soluble NSF attachment protein isoform X2	polymer	296	33290.7	4	UniProt (A0A8C2LIB4) Pfam (PF14938)	Cricetulus griseus (Chinese hamster)	N-ethylmaleimide sensitive fusion protein attachment protein alpha
5	O (O)	unknown sequence	polymer	13	1124.4	1		Cricetulus griseus (Chinese hamster)
6	AA, P, S, U, W (F, A, B, C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	5	Chemie (ADP)
7	Q, R, T, V, X... (A, B, C, D, E...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	7	Chemie (ATP)

Sequence modifications

A, B, C, D, E, F: 1 - 744 (UniProt: P18708)

PDB	External Database	Details
Gly -2	-	expression tag
Ala -1	-	expression tag
Asn 0	-	expression tag

J, I: 1 - 206 (UniProt: P60881)

PDB	External Database	Details
Met -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 85	Cys 85	conflict
Ala 88	Cys 88	conflict
Ala 90	Cys 90	conflict
Ala 92	Cys 92	conflict
Gly 207	-	linker

J, I: 208 - 502 (PDB: 9PD8)
K, L, M, N: 1 - 295 (UniProt: A0A8C2LIB4)

PDB	External Database	Details
Gly 0	-	expression tag
Ile 44	Met 44	conflict
Met 244	Val 244	conflict

Contents of the asymmetric unit

Polymers	Number of chains	15
	Total formula weight	810298.1
Non-Polymers*	Number of molecules	12
	Total formula weight	5686.3
All*	Total formula weight	815984.4

*Water molecules are not included.