9P99

CryoEM structure of integrin alphaEbeta7 bound to E-cadherin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Integrin alpha-E	polymer	1176	129950.2	1	UniProt (P38570) Pfam (PF00092) Pfam (PF01839) Pfam (PF20805)	Homo sapiens (human)	HML-1 antigen,Integrin alpha-IEL,Mucosal lymphocyte 1 antigen
2	B (B)	Integrin beta-7	polymer	776	84538.8	1	UniProt (P26010) Pfam (PF17205) Pfam (PF00362) Pfam (PF07974)	Homo sapiens (human)	Gut homing receptor beta subunit
3	C (C)	Cadherin-1	polymer	331	36625.7	1	UniProt (P12830) Pfam (PF00028)	Homo sapiens (human)	CAM 120/80,Epithelial cadherin,E-cadherin,Uvomorulin
4	D, E (D, E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	F (F)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
6	G, H, M (A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
7	I, J, K (A)	CALCIUM ION	non-polymer		40.1	3	Chemie (CA)
8	L, N, O, P (A, B)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)

Sequence modifications

A: -17 - 1105 (UniProt: P38570)

PDB	External Database	Details
Gly 1106	-	expression tag
Thr 1107	-	expression tag
Gly 1108	-	expression tag
Gly 1109	-	expression tag
Leu 1110	-	expression tag
Glu 1111	-	expression tag
Val 1112	-	expression tag
Leu 1113	-	expression tag
Phe 1114	-	expression tag
Gln 1115	-	expression tag
Gly 1116	-	expression tag
Pro 1117	-	expression tag
Gly 1118	-	expression tag
Glu 1119	-	expression tag
Asn 1120	-	expression tag
Ala 1121	-	expression tag
Gln 1122	-	expression tag
Cys 1123	-	expression tag
Glu 1124	-	expression tag
Lys 1125	-	expression tag
Glu 1126	-	expression tag
Leu 1127	-	expression tag
Gln 1128	-	expression tag
Ala 1129	-	expression tag
Leu 1130	-	expression tag
Glu 1131	-	expression tag
Lys 1132	-	expression tag
Glu 1133	-	expression tag
Asn 1134	-	expression tag
Ala 1135	-	expression tag
Gln 1136	-	expression tag
Leu 1137	-	expression tag
Glu 1138	-	expression tag
Trp 1139	-	expression tag
Glu 1140	-	expression tag
Leu 1141	-	expression tag
Gln 1142	-	expression tag
Ala 1143	-	expression tag
Leu 1144	-	expression tag
Glu 1145	-	expression tag
Lys 1146	-	expression tag
Glu 1147	-	expression tag
Leu 1148	-	expression tag
Ala 1149	-	expression tag
Gln 1150	-	expression tag
Trp 1151	-	expression tag
Ser 1152	-	expression tag
His 1153	-	expression tag
Pro 1154	-	expression tag
Gln 1155	-	expression tag
Phe 1156	-	expression tag
Glu 1157	-	expression tag
Lys 1158	-	expression tag

B: -18 - 704 (UniProt: P26010)

PDB	External Database	Details
Asp 705	-	expression tag
Thr 706	-	expression tag
Ser 707	-	expression tag
Gly 708	-	expression tag
Leu 709	-	expression tag
Glu 710	-	expression tag
Val 711	-	expression tag
Leu 712	-	expression tag
Phe 713	-	expression tag
Gln 714	-	expression tag
Gly 715	-	expression tag
Pro 716	-	expression tag
Gly 717	-	expression tag
Lys 718	-	expression tag
Asn 719	-	expression tag
Ala 720	-	expression tag
Gln 721	-	expression tag
Cys 722	-	expression tag
Lys 723	-	expression tag
Lys 724	-	expression tag
Lys 725	-	expression tag
Leu 726	-	expression tag
Gln 727	-	expression tag
Ala 728	-	expression tag
Leu 729	-	expression tag
Lys 730	-	expression tag
Lys 731	-	expression tag
Lys 732	-	expression tag
Asn 733	-	expression tag
Ala 734	-	expression tag
Gln 735	-	expression tag
Leu 736	-	expression tag
Lys 737	-	expression tag
Trp 738	-	expression tag
Lys 739	-	expression tag
Leu 740	-	expression tag
Gln 741	-	expression tag
Ala 742	-	expression tag
Leu 743	-	expression tag
Lys 744	-	expression tag
Lys 745	-	expression tag
Lys 746	-	expression tag
Leu 747	-	expression tag
Ala 748	-	expression tag
Gln 749	-	expression tag
Gly 750	-	expression tag
Gly 751	-	expression tag
His 752	-	expression tag
His 753	-	expression tag
His 754	-	expression tag
His 755	-	expression tag
His 756	-	expression tag
His 757	-	expression tag

C: 1 - 217 (UniProt: P12830)

PDB	External Database	Details
Met -113	-	initiating methionine
Ala -112	-	expression tag
Ser -111	-	expression tag
Met -110	-	expression tag
His -109	-	expression tag
His -108	-	expression tag
His -107	-	expression tag
His -106	-	expression tag
His -105	-	expression tag
His -104	-	expression tag
Gly -103	-	expression tag
Ser -102	-	expression tag
Ser -101	-	expression tag
Met -100	-	expression tag
Ala -99	-	expression tag
Ser -98	-	expression tag
Met -97	-	expression tag
Ser -96	-	expression tag
Asp -95	-	expression tag
Ser -94	-	expression tag
Glu -93	-	expression tag
Val -92	-	expression tag
Asn -91	-	expression tag
Gln -90	-	expression tag
Glu -89	-	expression tag
Ala -88	-	expression tag
Lys -87	-	expression tag
Pro -86	-	expression tag
Glu -85	-	expression tag
Val -84	-	expression tag
Lys -83	-	expression tag
Pro -82	-	expression tag
Glu -81	-	expression tag
Val -80	-	expression tag
Lys -79	-	expression tag
Pro -78	-	expression tag
Glu -77	-	expression tag
Thr -76	-	expression tag
His -75	-	expression tag
Ile -74	-	expression tag
Asn -73	-	expression tag
Leu -72	-	expression tag
Lys -71	-	expression tag
Val -70	-	expression tag
Ser -69	-	expression tag
Asp -68	-	expression tag
Gly -67	-	expression tag
Ser -66	-	expression tag
Ser -65	-	expression tag
Glu -64	-	expression tag
Ile -63	-	expression tag
Phe -62	-	expression tag
Phe -61	-	expression tag
Lys -60	-	expression tag
Ile -59	-	expression tag
Lys -58	-	expression tag
Lys -57	-	expression tag
Thr -56	-	expression tag
Thr -55	-	expression tag
Pro -54	-	expression tag
Leu -53	-	expression tag
Arg -52	-	expression tag
Arg -51	-	expression tag
Leu -50	-	expression tag
Met -49	-	expression tag
Glu -48	-	expression tag
Ala -47	-	expression tag
Phe -46	-	expression tag
Ala -45	-	expression tag
Lys -44	-	expression tag
Arg -43	-	expression tag
Gln -42	-	expression tag
Gly -41	-	expression tag
Lys -40	-	expression tag
Glu -39	-	expression tag
Met -38	-	expression tag
Asp -37	-	expression tag
Ser -36	-	expression tag
Leu -35	-	expression tag
Arg -34	-	expression tag
Phe -33	-	expression tag
Leu -32	-	expression tag
Tyr -31	-	expression tag
Asp -30	-	expression tag
Gly -29	-	expression tag
Ile -28	-	expression tag
Arg -27	-	expression tag
Ile -26	-	expression tag
Gln -25	-	expression tag
Ala -24	-	expression tag
Asp -23	-	expression tag
Gln -22	-	expression tag
Thr -21	-	expression tag
Pro -20	-	expression tag
Glu -19	-	expression tag
Asp -18	-	expression tag
Leu -17	-	expression tag
Asp -16	-	expression tag
Met -15	-	expression tag
Glu -14	-	expression tag
Asp -13	-	expression tag
Asn -12	-	expression tag
Asp -11	-	expression tag
Ile -10	-	expression tag
Ile -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
His -6	-	expression tag
Arg -5	-	expression tag
Glu -4	-	expression tag
Gln -3	-	expression tag
Ile -2	-	expression tag
Gly -1	-	expression tag
Gly 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	251114.7
Branched	Number of molecules	3
	Total formula weight	1597.5
Non-Polymers*	Number of molecules	10
	Total formula weight	1003.6
All*	Total formula weight	253715.8

*Water molecules are not included.