9P93

Cryo-EM structure of the PAC1sR-VIP-Gs complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (human)	Transducin beta chain 1
2	B (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (human)	G gamma-I
3	C (N)	Nanobody 35	polymer	138	15140.7	1		Lama glama
4	D (P)	Vasoactive intestinal peptide	polymer	28	3332.8	1	UniProt (P01282) Pfam (PF00123)	Homo sapiens	VIP,Vasoactive intestinal polypeptide
5	E (R)	Isoform S of Pituitary adenylate cyclase-activating polypeptide type I receptor	polymer	462	53155.9	1	UniProt (P41586) Pfam (PF02793) Pfam (PF00002)	Homo sapiens (human)	PAC1 receptor,PAC1R,PACAP type I receptor,PACAP-R-1,PACAP-R1
6	F (A)	Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	polymer	394	45699.4	1	UniProt (P63092) Pfam (PF00503)	Homo sapiens (human)	Adenylate cyclase-stimulating G alpha protein

Sequence modifications

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: 19 - 468 (UniProt: P41586)

PDB	External Database	Details
Asp 5	-	expression tag
Tyr 6	-	expression tag
Lys 7	-	expression tag
Asp 8	-	expression tag
Asp 9	-	expression tag
Asp 10	-	expression tag
Asp 11	-	expression tag
Leu 12	-	expression tag
Glu 13	-	expression tag
Val 14	-	expression tag
Leu 15	-	expression tag
Phe 16	-	expression tag
Gln 17	-	expression tag
Gly 18	-	expression tag
Pro 469	-	expression tag
Ala 470	-	expression tag
Gly 471	-	expression tag
Leu 472	-	expression tag
Glu 473	-	expression tag
Val 474	-	expression tag
Leu 475	-	expression tag
Phe 476	-	expression tag
Gln 477	-	expression tag
Gly 478	-	expression tag
Pro 479	-	expression tag
His 480	-	expression tag
His 481	-	expression tag
His 482	-	expression tag
His 483	-	expression tag
His 484	-	expression tag
His 485	-	expression tag
His 486	-	expression tag
His 487	-	expression tag

A: 1 - 394 (UniProt: P63092)

PDB	External Database	Details
Asn 54	Ser 54	engineered mutation
Ala 226	Gly 226	engineered mutation
Ala 268	Glu 268	engineered mutation
Lys 271	Asn 271	engineered mutation
Asp 274	Lys 274	engineered mutation
Lys 280	Arg 280	engineered mutation
Asp 284	Thr 284	engineered mutation
Thr 285	Ile 285	engineered mutation
Ser 366	Ala 366	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	163724.1
All*	Total formula weight	163724.1

*Water molecules are not included.