9P84

NTSR1-Gi-NTS(8-13) GTP-Bound Complex in the Non-Canonical, AHD Closed State (NC-Closed-GTP)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40415.0	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
2	B (R)	Neurotensin receptor type 1	polymer	435	48396.7	1	UniProt (P30989)	Homo sapiens (human)	NTR1,High-affinity levocabastine-insensitive neurotensin receptor,NTRH
3	C (L)	Neurotensin receptor type 1	polymer	6	819.0	1		Homo sapiens (human)
4	D (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	344	37671.1	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
5	E (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
6	F (A)	GUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (GTP)
7	G (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)

Sequence modifications

R: 20 - 418 (UniProt: P30989)

PDB	External Database	Details
Asp -19	-	expression tag
Tyr -18	-	expression tag
Lys -17	-	expression tag
Asp -16	-	expression tag
Asp -15	-	expression tag
Asp -14	-	expression tag
Asp -13	-	expression tag
Ala -12	-	expression tag
Met -11	-	expression tag
Gly -10	-	expression tag
Gln -9	-	expression tag
Pro -8	-	expression tag
Gly -7	-	expression tag
Asn -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Phe -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ala 1	-	expression tag
Pro 2	-	expression tag
Asn 3	-	expression tag
Arg 4	-	expression tag
Ser 5	-	expression tag
His 6	-	expression tag
Ala 7	-	expression tag
Pro 8	-	expression tag
Asp 9	-	expression tag
His 10	-	expression tag
Asp 11	-	expression tag
Val 12	-	expression tag
Glu 13	-	expression tag
Asn 14	-	expression tag
Leu 15	-	expression tag
Tyr 16	-	expression tag
Phe 17	-	expression tag
Gln 18	-	expression tag
Gly 19	-	expression tag
Leu 85	Ala 85	engineered mutation
-	Glu 282	deletion
-	His 283	deletion
-	Ser 284	deletion
-	Thr 285	deletion
-	Phe 286	deletion
-	Ser 287	deletion
-	Met 288	deletion
-	Ala 289	deletion
-	Ile 290	deletion
-	Glu 291	deletion
Leu 419	-	expression tag
Glu 420	-	expression tag
Val 421	-	expression tag
Leu 422	-	expression tag
Phe 423	-	expression tag
Gln 424	-	expression tag
Gly 425	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	135163.0
Non-Polymers*	Number of molecules	2
	Total formula weight	547.5
All*	Total formula weight	135710.5

*Water molecules are not included.