9P6P

Crystal Structure of the SARS-CoV-2 2'-O-Methyltransferase with (m7GpppA)pUpU (Cap-0) and S-Adenosyl-L-homocysteine (SAH).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	2'-O-methyltransferase	polymer	301	33627.5	2	UniProt (P0DTD1) Pfam (PF06460)	Severe acute respiratory syndrome coronavirus 2	Non-structural protein 16, nsp16
2	B, D (B, D)	Non-structural protein 10	polymer	142	15075.2	2	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2	nsp10, Growth factor-like peptide, GFL
3	E, F (E, G)	RNA (5'-D(*(M7G))-R(P*AP*UP*U)-3')	polymer	4	1336.8	2		Severe acute respiratory syndrome coronavirus 2
4	G, X (A, C)	S-ADENOSYL-L-HOMOCYSTEINE	non-polymer		384.4	2	Chemie (SAH)
5	AA, BA, CA, DA, H... (C, A, D)	CHLORIDE ION	non-polymer		35.5	11	Chemie (CL)
6	EA, FA, GA, HA, IA... (C, A, D, B)	SULFATE ION	non-polymer		96.1	19	Chemie (SO4)
7	LA, MA, T, U (D, B)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
8	Y (C)	7N-METHYL-8-HYDROGUANOSINE-5'-TRIPHOSPHATE	non-polymer		539.2	1	Chemie (MGT)
9	RA, SA, TA, UA, VA... (A, B, C, D, E...)	water	water		18.0	527	Chemie (HOH)

Sequence modifications

A, C: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B, D: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	100079.0
Non-Polymers*	Number of molecules	37
	Total formula weight	3784.9
All*	Total formula weight	103863.8

*Water molecules are not included.