9P3V

Zebrafish TRPM5 Q771A mutant with 5mM calcium and 0.5mM CBTA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	RNA-directed RNA polymerase L,Transient receptor potential cation channel subfamily M member 5	polymer	1475	166758.6	4	UniProt (A0A5P9VSM8) UniProt (S5UH55) Pfam (PF01353) Pfam (PF18139) Pfam (PF00520)	Danio rerio (zebrafish)	Large structural protein,Replicase,Transcriptase
2	E, K, Q, X (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
3	F, G, L, M, R... (A, B, C, D)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)
4	AA, H, N, T (D, A, B, C)	(10xi,22xi,25R)-5beta,13xi,14beta,17beta-spirostan-3beta-ol	non-polymer		416.6	4	Chemie (A1CGZ)
5	BA, I, O, U (D, A, B, C)	5-chloro-N-[(5-chloro-1,3-thiazol-2-yl)methyl]-1,2-benzothiazol-6-amine	non-polymer		316.2	4	Chemie (A1CGW)
6	J, P, V, W (A, B, C, D)	(2R)-2-(hydroxymethyl)-4-{[(10xi,13xi,22xi,25R)-5beta,14beta,17beta-spirostan-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside	non-polymer		843.0	4	Chemie (A1CG0)

Sequence modifications

A, B, C, D: -254 - -17 (UniProt: A0A5P9VSM8)

PDB	External Database	Details
Met -307	-	expression tag
His -306	-	expression tag
His -305	-	expression tag
His -304	-	expression tag
His -303	-	expression tag
His -302	-	expression tag
His -301	-	expression tag
His -300	-	expression tag
His -299	-	expression tag
Ala -298	-	expression tag
Ala -297	-	expression tag
Ala -296	-	expression tag
Ser -295	-	expression tag
Ala -294	-	expression tag
Trp -293	-	expression tag
Ser -292	-	expression tag
His -291	-	expression tag
Pro -290	-	expression tag
Gln -289	-	expression tag
Phe -288	-	expression tag
Glu -287	-	expression tag
Lys -286	-	expression tag
Gly -285	-	expression tag
Gly -284	-	expression tag
Gly -283	-	expression tag
Ser -282	-	expression tag
Gly -281	-	expression tag
Gly -280	-	expression tag
Gly -279	-	expression tag
Ser -278	-	expression tag
Gly -277	-	expression tag
Gly -276	-	expression tag
Ser -275	-	expression tag
Ala -274	-	expression tag
Trp -273	-	expression tag
Ser -272	-	expression tag
His -271	-	expression tag
Pro -270	-	expression tag
Gln -269	-	expression tag
Phe -268	-	expression tag
Glu -267	-	expression tag
Lys -266	-	expression tag
Gly -265	-	expression tag
Gly -264	-	expression tag
Gly -263	-	expression tag
Ser -262	-	expression tag
Gly -261	-	expression tag
Gly -260	-	expression tag
Gly -259	-	expression tag
Ser -258	-	expression tag
Gly -257	-	expression tag
Gly -256	-	expression tag
Met -255	-	expression tag
Ser -16	-	linker
Gly -15	-	linker
Leu -14	-	linker
Arg -13	-	linker
Ser -12	-	linker
Gly -11	-	linker
Leu -10	-	linker
Val -9	-	linker
Pro -8	-	linker
Arg -7	-	linker
Gly -6	-	linker
Ser -5	-	linker
Gly -4	-	linker
Gly -3	-	linker
Arg -2	-	linker
Ala -1	-	linker
Thr 0	-	linker

A, B, C, D: 1 - 1165 (UniProt: S5UH55)

PDB	External Database	Details
Ala 771	Gln 771	engineered mutation
Gly 1149	Ala 1149	conflict
Phe 1166	-	expression tag
Glu 1167	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	667034.5
Non-Polymers*	Number of molecules	24
	Total formula weight	7509.1
All*	Total formula weight	674543.6

*Water molecules are not included.