9P3P

Zebrafish TRPM5 with 5mM EGTA and 0.5mM CBTA

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Green fluorescent protein,Transient receptor potential cation channel subfamily M member 5	polymer	1475	166815.7	4	UniProt (P42212) UniProt (S5UH55) Pfam (PF01353) Pfam (PF18139) Pfam (PF00520)	Danio rerio (zebrafish)
2	E, I, M, Q (A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	4	Chemie (NAG)
3	F, J, N, R (A, B, C, D)	(2R)-2-(hydroxymethyl)-4-{[(25R)-10alpha,14beta,17beta-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside	non-polymer		841.0	4	Chemie (YUY)
4	G, K, O, S (A, B, C, D)	(25R)-14beta,17beta-spirost-5-en-3beta-ol	non-polymer		414.6	4	Chemie (YUV)
5	H, L, P, T (A, B, C, D)	5-chloro-N-[(5-chloro-1,3-thiazol-2-yl)methyl]-1,2-benzothiazol-6-amine	non-polymer		316.2	4	Chemie (A1CGW)

Sequence modifications

A, B, C, D: -253 - -17 (UniProt: P42212)

PDB	External Database	Details
Met -307	-	initiating methionine
His -306	-	expression tag
His -305	-	expression tag
His -304	-	expression tag
His -303	-	expression tag
His -302	-	expression tag
His -301	-	expression tag
His -300	-	expression tag
His -299	-	expression tag
Ala -298	-	expression tag
Ala -297	-	expression tag
Ala -296	-	expression tag
Ser -295	-	expression tag
Ala -294	-	expression tag
Trp -293	-	expression tag
Ser -292	-	expression tag
His -291	-	expression tag
Pro -290	-	expression tag
Gln -289	-	expression tag
Phe -288	-	expression tag
Glu -287	-	expression tag
Lys -286	-	expression tag
Gly -285	-	expression tag
Gly -284	-	expression tag
Gly -283	-	expression tag
Ser -282	-	expression tag
Gly -281	-	expression tag
Gly -280	-	expression tag
Gly -279	-	expression tag
Ser -278	-	expression tag
Gly -277	-	expression tag
Gly -276	-	expression tag
Ser -275	-	expression tag
Ala -274	-	expression tag
Trp -273	-	expression tag
Ser -272	-	expression tag
His -271	-	expression tag
Pro -270	-	expression tag
Gln -269	-	expression tag
Phe -268	-	expression tag
Glu -267	-	expression tag
Lys -266	-	expression tag
Gly -265	-	expression tag
Gly -264	-	expression tag
Gly -263	-	expression tag
Ser -262	-	expression tag
Gly -261	-	expression tag
Gly -260	-	expression tag
Gly -259	-	expression tag
Ser -258	-	expression tag
Gly -257	-	expression tag
Gly -256	-	expression tag
Met -255	-	expression tag
Val -254	-	expression tag
Leu -191	Phe 64	conflict
Thr -190	Ser 65	conflict
Lys -49	Ala 206	conflict
Leu -24	His 231	conflict
Ser -16	-	linker
Gly -15	-	linker
Leu -14	-	linker
Arg -13	-	linker
Ser -12	-	linker
Gly -11	-	linker
Leu -10	-	linker
Val -9	-	linker
Pro -8	-	linker
Arg -7	-	linker
Gly -6	-	linker
Ser -5	-	linker
Gly -4	-	linker
Gly -3	-	linker
Arg -2	-	linker
Ala -1	-	linker
Thr 0	-	linker

A, B, C, D: 1 - 1165 (UniProt: S5UH55)

PDB	External Database	Details
Gly 1149	Ala 1149	conflict
Phe 1166	-	expression tag
Glu 1167	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	667262.7
Non-Polymers*	Number of molecules	16
	Total formula weight	7172.4
All*	Total formula weight	674435.1

*Water molecules are not included.