9OXA

CryoEM structure of Gi-coupled TAS2R43 with aristolochic acid I

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Soluble cytochrome b562,Taste receptor type 2 member 43	polymer	645	72130.3	1	UniProt (P0ABE7) UniProt (P59537)	Homo sapiens (human)	Cytochrome b-562,T2R43,Taste receptor type 2 member 52,T2R52
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	402	42936.7	1	UniProt (P62873)	Homo sapiens (human)	Transducin beta chain 1
3	C (C)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768)	Homo sapiens (human)	G gamma-I
4	D (A)	Guanine nucleotide-binding protein G(i) subunit alpha-1	polymer	354	40414.0	1	UniProt (P63096)	Homo sapiens (human)	Adenylate cyclase-inhibiting G alpha protein
5	E (D)	scFv16	polymer	267	28668.9	1		Mus musculus (mouse)
6	F (R)	8-methoxy-6-nitro-naphtho[1,2-e][1,3]benzodioxole-5-carboxylic acid	non-polymer		341.3	1	Chemie (GOQ) PubChem (2236)
7	G (R)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

R: -116 - -12 (UniProt: P0ABE7)

PDB	External Database	Details
Asp -150	-	expression tag
Tyr -149	-	expression tag
Lys -148	-	expression tag
Asp -147	-	expression tag
Asp -146	-	expression tag
Asp -145	-	expression tag
Asp -144	-	expression tag
Ala -143	-	expression tag
Lys -142	-	expression tag
Leu -141	-	expression tag
Gln -140	-	expression tag
Thr -139	-	expression tag
Met -138	-	expression tag
His -137	-	expression tag
His -136	-	expression tag
His -135	-	expression tag
His -134	-	expression tag
His -133	-	expression tag
His -132	-	expression tag
His -131	-	expression tag
His -130	-	expression tag
His -129	-	expression tag
His -128	-	expression tag
Glu -127	-	expression tag
Asn -126	-	expression tag
Leu -125	-	expression tag
Tyr -124	-	expression tag
Phe -123	-	expression tag
Gln -122	-	expression tag
Gly -121	-	expression tag
Gly -120	-	expression tag
Thr -119	-	expression tag
Thr -118	-	expression tag
Met -117	-	expression tag
Trp -110	Met 29	conflict
Ile -15	His 124	conflict
Leu -11	-	linker
Gly -10	-	linker
Ser -9	-	linker
Thr -8	-	linker
Leu -7	-	linker
Glu -6	-	linker
Val -5	-	linker
Leu -4	-	linker
Phe -3	-	linker
Gln -2	-	linker
Gly -1	-	linker
Pro 0	-	linker

R: 1 - 309 (UniProt: P59537)
R: 310 - 494 (PDB: 9OXA)
B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Leu -10	-	expression tag
Glu -9	-	expression tag
Val -8	-	expression tag
Leu -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag
Gly 341	-	expression tag
Gly 342	-	expression tag
Ser 343	-	expression tag
Gly 344	-	expression tag
Gly 345	-	expression tag
Gly 346	-	expression tag
Gly 347	-	expression tag
Ser 348	-	expression tag
Gly 349	-	expression tag
Gly 350	-	expression tag
Ser 351	-	expression tag
Ser 352	-	expression tag
Ser 353	-	expression tag
Gly 354	-	expression tag
Gly 355	-	expression tag
Gly 356	-	expression tag
Gly 357	-	expression tag
Ser 358	-	expression tag
Gly 359	-	expression tag
Gly 360	-	expression tag
Gly 361	-	expression tag
Gly 362	-	expression tag
Ser 363	-	expression tag
Gly 364	-	expression tag
Gly 365	-	expression tag
Ser 366	-	expression tag
Ser 367	-	expression tag
Ser 368	-	expression tag
Gly 369	-	expression tag
Gly 370	-	expression tag
Val 371	-	expression tag
Ser 372	-	expression tag
Gly 373	-	expression tag
Trp 374	-	expression tag
Arg 375	-	expression tag
Leu 376	-	expression tag
Phe 377	-	expression tag
Lys 378	-	expression tag
Lys 379	-	expression tag
Ile 380	-	expression tag
Ser 381	-	expression tag
Gly 382	-	expression tag
Gly 383	-	expression tag
Ser 384	-	expression tag

A: 1 - 354 (UniProt: P63096)

PDB	External Database	Details
Asn 47	Ser 47	engineered mutation
Ala 203	Gly 203	engineered mutation
Ala 245	Glu 245	engineered mutation
Ser 326	Ala 326	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	192011.1
Non-Polymers*	Number of molecules	1
	Total formula weight	341.3
All*	Total formula weight	192352.4

*Water molecules are not included.