9ON0

Crystal structure of E. coli ApaH D37A mutant in complex with Ap4U

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]	polymer	286	32116.5	2	UniProt (P05637) Pfam (PF00149)	Escherichia coli K-12	Ap4A hydrolase,Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase,Diadenosine tetraphosphatase
2	C, F (A, B)	P1-(5'-Adenosyl) P4-(5'-uridyl) tetraphosphate	non-polymer		813.3	2	Chemie (A1L89)
3	D, G (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
4	E (B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
5	H, I (A, B)	water	water		18.0	565	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: P05637)

PDB	External Database	Details
Ala 37	Asp 37	engineered mutation
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64233.0
Non-Polymers*	Number of molecules	5
	Total formula weight	1974.9
All*	Total formula weight	66207.9

*Water molecules are not included.