9OMA

Cryo-EM structure of PCMTD1-ELOBC-CUL5-RBX2 (CRL5-PCMTD1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein-L-isoaspartate O-methyltransferase domain-containing protein 1	polymer	358	40814.3	1	UniProt (Q96MG8) Pfam (PF01135)	Homo sapiens (human)
2	B (B)	Cullin-5	polymer	783	91436.7	1	UniProt (Q93034) Pfam (PF00888) Pfam (PF26557) Pfam (PF10557)	Homo sapiens (human)	CUL-5,Vasopressin-activated calcium-mobilizing receptor 1,VACM-1
3	C (C)	RING-box protein 2	polymer	113	12721.5	1	UniProt (Q9UBF6) Pfam (PF12678)	Homo sapiens (human)	Rbx2,CKII beta-binding protein 1,CKBBP1,RING finger protein 7,Regulator of cullins 2,Sensitive to apoptosis gene protein
4	D (D)	Elongin-B	polymer	118	13147.8	1	UniProt (Q15370) Pfam (PF00240)	Homo sapiens (human)	EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2
5	E (E)	Elongin-C	polymer	96	10843.4	1	UniProt (Q15369) Pfam (PF03931)	Homo sapiens (human)	EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1

Sequence modifications

A: 1 - 357 (UniProt: Q96MG8)

PDB	External Database	Details
Ser 0	-	expression tag
Ile 312	Asn 312	variant

B: 1 - 780 (UniProt: Q93034)

PDB	External Database	Details
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Tyr 567	Ser 567	conflict
Val 619	Leu 619	conflict
Leu 767	Arg 767	conflict

C: 1 - 113 (UniProt: Q9UBF6)

PDB	External Database	Details
Pro 10	Thr 10	conflict
Val 12	Ala 12	conflict
Ser 14	Ala 14	conflict
Ala 20	Ser 20	conflict

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	168963.7
All*	Total formula weight	168963.7

*Water molecules are not included.