9OM4

Cryo-EM structure of filament form Acidithiobacillus caldus (Aca) short prokaryotic argonautes, HNH-associated (SPARHA) with gRNA and tDNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I, M (A, E, I, M)	HNH endonuclease	polymer	435	50263.3	4		Acidithiobacillus caldus
2	B, F, J, N (B, F, J, N)	Prokaryotic argonaute MID-PIWI family protein	polymer	471	53150.6	4		Acidithiobacillus caldus
3	C, G, K, O (C, G, K, O)	RNA (5'-R(P*AP*UP*UP*UP*GP*GP*UP*UP*GP*CP*GP*GP*UP*GP*CP*UP*CP*U)-3')	polymer	18	5720.4	4		Acidithiobacillus caldus
4	D, H, L, P (D, H, L, P)	DNA (5'-D(*AP*GP*AP*GP*CP*AP*CP*CP*GP*CP*AP*AP*CP*CP*AP*AP*AP*T)-3')	polymer	18	5487.6	4		Acidithiobacillus caldus
5	Q, R, S, T (B, F, J, N)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	458487.7
Non-Polymers*	Number of molecules	4
	Total formula weight	219.8
All*	Total formula weight	458707.4

*Water molecules are not included.