9OLY

Crystal structure of E. coli ApaH D37A mutant, apo form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Bis(5'-nucleosyl)-tetraphosphatase [symmetrical]	polymer	286	32116.5	2	UniProt (P05637) Pfam (PF00149)	Escherichia coli K-12	Ap4A hydrolase,Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase,Diadenosine tetraphosphatase
2	C, G (A, B)	MANGANESE (II) ION	non-polymer		54.9	2	Chemie (MN)
3	D, E, H, I, J (A, B)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
4	F (B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
5	K, L (A, B)	water	water		18.0	253	Chemie (HOH)

Sequence modifications

A, B: 1 - 280 (UniProt: P05637)

PDB	External Database	Details
Ala 37	Asp 37	engineered mutation
His 281	-	expression tag
His 282	-	expression tag
His 283	-	expression tag
His 284	-	expression tag
His 285	-	expression tag
His 286	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	64233.0
Non-Polymers*	Number of molecules	8
	Total formula weight	828.5
All*	Total formula weight	65061.5

*Water molecules are not included.