9OGM

BG505 MD39.3 Env gp151 MPER nanodisc in complex with 10E8, BG18 and VRC01 Fabs (1x 10E8 Fab)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, F (D, F, I)	BG18 Fab heavy chain	polymer	233	25043.2	3		Homo sapiens
2	B, D, G (E, G, J)	BG18 Fab light chain	polymer	214	22937.4	3		Homo sapiens
3	E (H)	10E8 Fab heavy chain	polymer	236	25447.5	1		Homo sapiens
4	H, K, L (K, N, P)	VRC01 Fab heavy chain	polymer	224	24367.6	3		Homo sapiens
5	I (L)	10E8 Fab light chain	polymer	215	22989.5	1		Homo sapiens
6	J, M, Q (M, Q, O)	VRC01 Fab light chain	polymer	210	23172.7	3		Homo sapiens
7	N, O, P (A, B, C)	Envelope glycoprotein gp160	polymer	735	81468.1	3	UniProt (Q2N0S6) UniProt (A0A6H1VGN1)	Human immunodeficiency virus 1	Env polyprotein
8	R (R)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	1	In PDB GlyTouCan (G15407YE)
9	S (S)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
10	T, Z (T, Z)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	2	In PDB GlyTouCan (G40702WU)
11	U (U)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G16404YH)
12	AA, CA, GA, V, W (a, c, g, V, W)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
13	EA, X (e, X)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	2	In PDB GlyTouCan (G22768VO)
14	Y (Y)	alpha-D-mannopyranose-(1-2)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G99516QU)
15	BA, DA (b, d)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
16	FA (f)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1721.5	1	In PDB GlyTouCan (G91704UR)
17	HA (h)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1073.0	1	In PDB GlyTouCan (G01760ZU)
18	AB, BB, CB, DB, EB... (B, C, D, F, I...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	30	Chemie (NAG)

Sequence modifications

A, B, C: 31 - 503 (UniProt: Q2N0S6)

PDB	External Database	Details
Thr 29	-	expression tag
Gly 30	-	expression tag
Ile 90	Thr 89	conflict
Glu 106	Thr 105	conflict
Ile 271	Met 270	conflict
Leu 288	Phe 287	conflict
Val 304	Arg 303	conflict
Tyr 319	Ala 316	conflict
Asn 332	Thr 330	conflict
Gln 363	Asn 361	conflict
Cys 501	Ala 498	conflict
Ser 503	-	linker
His 503	-	linker
Ser 503	-	linker
Gly 503	-	linker
Ser 503	-	linker
Gly 503	-	linker
Gly 503	-	linker
Ser 503	-	linker
Gly 503	-	linker
Ser 503	-	linker
Gly 503	-	linker
Gly 503	-	linker
His 503	-	linker
Ala 503	-	linker

A, B, C: 503 - 709 (UniProt: A0A6H1VGN1)

PDB	External Database	Details
Ser 503	Phe 516	conflict
Pro 559	Ile 556	conflict
Pro 561	Ala 558	conflict
Asp 568	Leu 565	conflict
His 570	Val 567	conflict
His 585	Arg 582	conflict
Cys 605	Thr 602	conflict
Asp 618	Asn 615	conflict
Lys 625	Asn 622	conflict
Thr 676	Ser 673	conflict
Ser 696	Arg 693	conflict
Gly 710	-	expression tag
Ser 711	-	expression tag
Gly 712	-	expression tag
Gly 713	-	expression tag
Ser 714	-	expression tag
Gly 715	-	expression tag
Leu 716	-	expression tag
Glu 717	-	expression tag
Val 718	-	expression tag
Leu 719	-	expression tag
Phe 720	-	expression tag
Gln 721	-	expression tag
Gly 722	-	expression tag
Pro 723	-	expression tag
Gly 724	-	expression tag
Ser 725	-	expression tag
Leu 726	-	expression tag
Glu 727	-	expression tag
Trp 728	-	expression tag
Ser 729	-	expression tag
His 730	-	expression tag
Pro 731	-	expression tag
Gln 732	-	expression tag
Phe 733	-	expression tag
Glu 734	-	expression tag
Lys 735	-	expression tag
Gly 736	-	expression tag
Gly 737	-	expression tag
Gly 738	-	expression tag
Ser 739	-	expression tag
Gly 740	-	expression tag
Gly 741	-	expression tag
Gly 742	-	expression tag
Ser 743	-	expression tag
Gly 744	-	expression tag
Gly 745	-	expression tag
Gly 746	-	expression tag
Ser 747	-	expression tag
Trp 748	-	expression tag
Ser 749	-	expression tag
His 750	-	expression tag
Pro 751	-	expression tag
Gln 752	-	expression tag
Phe 753	-	expression tag
Glu 754	-	expression tag
Lys 755	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	579403.8
Branched	Number of molecules	17
	Total formula weight	15484.0
Non-Polymers*	Number of molecules	30
	Total formula weight	6636.2
All*	Total formula weight	601524.0

*Water molecules are not included.