9OGB
Cryo-EM structure of human exportin-1 conjugated with selinexor and bound to yeast RAN-GTP and human ASB8-ELOB/C
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Exportin-1 | polymer | 1073 | 123662.5 | 1 | UniProt (O14980) Pfam (PF03810) Pfam (PF08389) Pfam (PF18777) Pfam (PF18784) Pfam (PF18787) Pfam (PF08767) | Homo sapiens (human) | Exp1,Chromosome region maintenance 1 protein homolog |
| 2 | B (B) | GTP-binding nuclear protein GSP1/CNR1 | polymer | 181 | 20561.7 | 1 | UniProt (P32835) Pfam (PF00071) | Saccharomyces cerevisiae (brewer's yeast) | Chromosome stability protein 17,GTPase Ran homolog,Genetic suppressor of PRP20-1 |
| 3 | C (C) | Ankyrin repeat and SOCS box protein 8 | polymer | 274 | 29869.2 | 1 | UniProt (Q9H765) Pfam (PF07525) Pfam (PF12796) | Homo sapiens (human) | ASB-8 |
| 4 | D (E) | Elongin-C | polymer | 97 | 10974.6 | 1 | UniProt (Q15369) Pfam (PF03931) | Homo sapiens (human) | EloC,Elongin 15 kDa subunit,RNA polymerase II transcription factor SIII subunit C,SIII p15,Transcription elongation factor B polypeptide 1 |
| 5 | E (F) | Elongin-B | polymer | 118 | 13147.8 | 1 | UniProt (Q15370) Pfam (PF00240) | Homo sapiens (human) | EloB,Elongin 18 kDa subunit,RNA polymerase II transcription factor SIII subunit B,SIII p18,Transcription elongation factor B polypeptide 2 |
| 6 | F (A) | selinexor, bound form | non-polymer | 445.3 | 1 | Chemie (V6A) | |||
| 7 | G (B) | GUANOSINE-5'-TRIPHOSPHATE | non-polymer | 523.2 | 1 | Chemie (GTP) | |||
| 8 | H (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) |
Sequence modifications
A: 1 - 1071 (UniProt: O14980)
B: 1 - 179 (UniProt: P32835)
C: 17 - 288 (UniProt: Q9H765)
E: 17 - 112 (UniProt: Q15369)
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Leu 71 | Gln 71 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 15 | - | expression tag |
| Ser 16 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 16 | - | initiating methionine |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 198215.8 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 992.8 | |
| All* | Total formula weight | 199208.6 |
