9OC4
High-resolution cryo-EM structure of KdpFABC in the E1P-ADP state in lipid nanodisc
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Potassium-transporting ATPase potassium-binding subunit | polymer | 557 | 59218.6 | 1 | UniProt (P03959) | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] A chain,Potassium-binding and translocating subunit A,Potassium-translocating ATPase A chain |
| 2 | B (B) | Potassium-transporting ATPase ATP-binding subunit | polymer | 682 | 72347.8 | 1 | UniProt (P03960) | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] B chain,Potassium-binding and translocating subunit B,Potassium-translocating ATPase B chain |
| 3 | C (C) | Potassium-transporting ATPase KdpC subunit | polymer | 208 | 22299.2 | 1 | UniProt (P03961) | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] C chain,Potassium-binding and translocating subunit C,Potassium-translocating ATPase C chain |
| 4 | D (D) | Potassium-transporting ATPase KdpF subunit | polymer | 29 | 3071.7 | 1 | UniProt (P36937) | Escherichia coli K-12 | ATP phosphohydrolase [potassium-transporting] F chain,Potassium-binding and translocating subunit F,Potassium-translocating ATPase F chain |
| 5 | E, J (A, B) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) | |||
| 6 | F, G (A, B) | (2R)-3-(((2-aminoethoxy)(hydroxy)phosphoryl)oxy)-2-(palmitoyloxy)propyl (E)-octadec-9-enoate | non-polymer | 718.0 | 2 | Chemie (9Y0) | |||
| 7 | H (B) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 8 | I, K (B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | L, M, N (A, B, C) | water | water | 18.0 | 90 | Chemie (HOH) |
Sequence modifications
C: 1 - 190 (UniProt: P03961)
| PDB | External Database | Details |
|---|---|---|
| Gly 191 | - | expression tag |
| Thr 192 | - | expression tag |
| Gly 193 | - | expression tag |
| Leu 194 | - | expression tag |
| Val 195 | - | expression tag |
| Pro 196 | - | expression tag |
| Arg 197 | - | expression tag |
| Gly 198 | - | expression tag |
| Ser 199 | - | expression tag |
| Ser 200 | - | expression tag |
| His 201 | - | expression tag |
| His 202 | - | expression tag |
| His 203 | - | expression tag |
| His 204 | - | expression tag |
| His 205 | - | expression tag |
| His 206 | - | expression tag |
| His 207 | - | expression tag |
| His 208 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 156937.4 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 1990.0 | |
| All* | Total formula weight | 158927.4 |
