9OAL

Cryo-EM structure of EBV gB prefusion construct C3-GT

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Envelope glycoprotein B	polymer	775	85973.3	3	UniProt (P0C762)	human gammaherpesvirus 4 (Epstein-Barr virus)	gB
2	D, E, F (D, E, F)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	3	In PDB GlyTouCan (G15407YE)

Sequence modifications

A, B, C: 1 - 688 (UniProt: P0C762)

PDB	External Database	Details
Cys 89	Ile 89	engineered mutation
His 112	Trp 112	engineered mutation
Arg 113	Tyr 113	engineered mutation
Glu 128	Asp 128	conflict
Cys 175	Ala 175	engineered mutation
Arg 193	Trp 193	engineered mutation
Val 194	Leu 194	engineered mutation
Glu 195	Ile 195	engineered mutation
Ala 196	Trp 196	engineered mutation
Glu 220	Asp 220	engineered mutation
Thr 241	Lys 241	conflict
Ile 316	His 316	engineered mutation
Gln 320	Asp 320	engineered mutation
Leu 325	Ser 325	engineered mutation
Gly 428	Arg 428	engineered mutation
Gly 429	Arg 429	engineered mutation
Ser 430	Arg 430	conflict
Gly 431	Arg 431	engineered mutation
Gly 432	Arg 432	engineered mutation
Asn 433	Asp 433	conflict
Gly 628	-	insertion
Cys 628	-	insertion
Gly 628	Leu 628	conflict
Cys 634	Glu 634	engineered mutation
Gly 689	-	expression tag
Gly 690	-	expression tag
Ser 691	-	expression tag
Gly 692	-	expression tag
Tyr 693	-	expression tag
Ile 694	-	expression tag
Pro 695	-	expression tag
Glu 696	-	expression tag
Ala 697	-	expression tag
Pro 698	-	expression tag
Arg 699	-	expression tag
Asp 700	-	expression tag
Gly 701	-	expression tag
Gln 702	-	expression tag
Ala 703	-	expression tag
Tyr 704	-	expression tag
Val 705	-	expression tag
Arg 706	-	expression tag
Lys 707	-	expression tag
Asp 708	-	expression tag
Gly 709	-	expression tag
Glu 710	-	expression tag
Trp 711	-	expression tag
Val 712	-	expression tag
Leu 713	-	expression tag
Leu 714	-	expression tag
Ser 715	-	expression tag
Thr 716	-	expression tag
Phe 717	-	expression tag
Leu 718	-	expression tag
Gly 719	-	expression tag
Arg 720	-	expression tag
Ala 721	-	expression tag
Ala 722	-	expression tag
Ala 723	-	expression tag
Ser 724	-	expression tag
Ser 725	-	expression tag
Leu 726	-	expression tag
Glu 727	-	expression tag
Val 728	-	expression tag
Leu 729	-	expression tag
Phe 730	-	expression tag
Gln 731	-	expression tag
Gly 732	-	expression tag
Pro 733	-	expression tag
Gly 734	-	expression tag
His 735	-	expression tag
His 736	-	expression tag
His 737	-	expression tag
His 738	-	expression tag
His 739	-	expression tag
His 740	-	expression tag
His 741	-	expression tag
His 742	-	expression tag
Ser 743	-	expression tag
Ala 744	-	expression tag
Trp 745	-	expression tag
Ser 746	-	expression tag
His 747	-	expression tag
Pro 748	-	expression tag
Gln 749	-	expression tag
Phe 750	-	expression tag
Glu 751	-	expression tag
Lys 752	-	expression tag
Gly 753	-	expression tag
Gly 754	-	expression tag
Gly 755	-	expression tag
Ser 756	-	expression tag
Gly 757	-	expression tag
Gly 758	-	expression tag
Gly 759	-	expression tag
Gly 760	-	expression tag
Ser 761	-	expression tag
Gly 762	-	expression tag
Gly 763	-	expression tag
Ser 764	-	expression tag
Ala 765	-	expression tag
Trp 766	-	expression tag
Ser 767	-	expression tag
His 768	-	expression tag
Pro 769	-	expression tag
Gln 770	-	expression tag
Phe 771	-	expression tag
Glu 772	-	expression tag
Lys 773	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	257919.8
Branched	Number of molecules	3
	Total formula weight	1759.6
All*	Total formula weight	259679.4

*Water molecules are not included.