9O7O
CryoEM structure of M. mazei topoisomerase VI(A-E342Q)-minicircle DNA complex in asymmetric state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Type 2 DNA topoisomerase 6 subunit A | polymer | 369 | 42178.3 | 2 | UniProt (Q8PUB7) | Methanosarcina mazei Go1 | Type II DNA topoisomerase VI subunit A |
| 2 | B, D (B, D) | Type 2 DNA topoisomerase 6 subunit B | polymer | 621 | 68853.4 | 2 | UniProt (Q8PUB8) | Methanosarcina mazei Go1 | Type II DNA topoisomerase VI subunit B,TopoVI-B |
| 3 | E, F (F, H) | Minicircle DNA (arbitrary model sequence) | polymer | 74 | 22798.8 | 2 | unidentified | ||
| 4 | G, J (B, D) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
| 5 | H, K (B, D) | CALCIUM ION | non-polymer | 40.1 | 2 | Chemie (CA) | |||
| 6 | I, L (B, D) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) |
Sequence modifications
A, C: 1 - 369 (UniProt: Q8PUB7)
| PDB | External Database | Details |
|---|---|---|
| Gln 342 | Glu 342 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 267661.0 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1170.7 | |
| All* | Total formula weight | 268831.7 |






