9O68

Crystal Structure of Tryptophanyl-tRNA synthetase from Klebsiella aerogenes (tryptophan bound)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Tryptophan--tRNA ligase	polymer	355	39766.2	2	UniProt (A0A0H3FKK2) Pfam (PF00579)	Klebsiella aerogenes KCTC 2190	Tryptophanyl-tRNA synthetase,TrpRS
2	C, H (A, B)	TRYPTOPHAN	non-polymer		204.2	2	Chemie (TRP)
3	D, E, I (A, B)	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	F (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	G, J (A, B)	TETRAETHYLENE GLYCOL	non-polymer		194.2	2	Chemie (PG4)
6	K, L (A, B)	water	water		18.0	148	Chemie (HOH)

Sequence modifications

A, B: 1 - 334 (UniProt: A0A0H3FKK2)

PDB	External Database	Details
Met -20	-	initiating methionine
Ala -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Met -12	-	expression tag
Gly -11	-	expression tag
Thr -10	-	expression tag
Leu -9	-	expression tag
Glu -8	-	expression tag
Ala -7	-	expression tag
Gln -6	-	expression tag
Thr -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	79532.3
Non-Polymers*	Number of molecules	8
	Total formula weight	999.3
All*	Total formula weight	80531.6

*Water molecules are not included.