9O65
Cryo-EM structure of SHOC2-KRAS-PP1CA (SKP) complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Leucine-rich repeat protein SHOC-2 | polymer | 582 | 64954.8 | 1 | UniProt (Q9UQ13) | Homo sapiens (human) | Protein soc-2 homolog,Protein sur-8 homolog |
| 2 | B (B) | Isoform 2B of GTPase KRas | polymer | 169 | 19300.8 | 1 | UniProt (P01116) | Homo sapiens (human) | K-Ras 2,Ki-Ras,c-K-ras,c-Ki-ras |
| 3 | C (C) | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | polymer | 330 | 37544.0 | 1 | UniProt (P62136) | Homo sapiens (human) | PP-1A |
| 4 | D (B) | PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER | non-polymer | 522.2 | 1 | Chemie (GNP) | |||
| 5 | E (B) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 6 | F, G (C) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) |
Sequence modifications
A: 1 - 582 (UniProt: Q9UQ13)
B: 1 - 169 (UniProt: P01116)
C: 2 - 330 (UniProt: P62136)
| PDB | External Database | Details |
|---|---|---|
| Ile 173 | Met 173 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 61 | Gln 61 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Arg 50 | Pro 50 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 121799.6 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 656.4 | |
| All* | Total formula weight | 122456.0 |
