9O4N

Cryo-EM structure of CR12042 Fab in complex with influenza virus neuraminidase from A/California/07/2009 (H1N1)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, B, C, D)	Neuraminidase	polymer	478	52806.2	4	UniProt (C7FH46) Pfam (PF00064) UniProt (by SIFTS) (C4RT50)	Influenza A virus (A/California/07/2009(H1N1))
2	B, E, H, K (H, E, F, G)	CR12042 heavy chain	polymer	125	13702.0	4		Homo sapiens
3	C, F, I, L (L, I, J, K)	CR12042 light chain	polymer	112	12120.6	4		Homo sapiens
4	M, N, P, Q, S... (M, N, P, Q, S...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	8	In PDB GlyTouCan (G42666HT)
5	O, R, U, X (O, R, U, X)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	4	In PDB GlyTouCan (G21290RB)
6	AA, BA, CA, DA, EA... (A, B, C, D)	CALCIUM ION	non-polymer		40.1	9	Chemie (CA)

Sequence modifications

A, B, C, D: 83 - 470 (UniProt: C7FH46)

PDB	External Database	Details
Met -8	-	initiating methionine
Tyr -7	-	expression tag
Ser -6	-	expression tag
Met -5	-	expression tag
Gln -4	-	expression tag
Leu -3	-	expression tag
Ala -2	-	expression tag
Ser -1	-	expression tag
Cys 0	-	expression tag
Val 1	-	expression tag
Thr 2	-	expression tag
Leu 3	-	expression tag
Thr 4	-	expression tag
Leu 5	-	expression tag
Val 6	-	expression tag
Leu 7	-	expression tag
Leu 8	-	expression tag
Val 9	-	expression tag
Asn 10	-	expression tag
Ser 11	-	expression tag
Gln 12	-	expression tag
His 13	-	expression tag
His 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
His 18	-	expression tag
Gly 19	-	expression tag
Ser 20	-	expression tag
Ala 21	-	expression tag
Trp 22	-	expression tag
Ser 23	-	expression tag
His 24	-	expression tag
Pro 25	-	expression tag
Gln 26	-	expression tag
Phe 27	-	expression tag
Glu 28	-	expression tag
Lys 29	-	expression tag
Gly 30	-	expression tag
Gly 31	-	expression tag
Ser 32	-	expression tag
Ser 33	-	expression tag
Ser 34	-	expression tag
Asp 35	-	expression tag
Tyr 36	-	expression tag
Ser 37	-	expression tag
Asp 38	-	expression tag
Leu 39	-	expression tag
Gln 40	-	expression tag
Arg 41	-	expression tag
Val 42	-	expression tag
Lys 43	-	expression tag
Gln 44	-	expression tag
Glu 45	-	expression tag
Leu 46	-	expression tag
Leu 47	-	expression tag
Glu 48	-	expression tag
Glu 49	-	expression tag
Val 50	-	expression tag
Lys 51	-	expression tag
Lys 52	-	expression tag
Glu 53	-	expression tag
Leu 54	-	expression tag
Gln 55	-	expression tag
Lys 56	-	expression tag
Val 57	-	expression tag
Lys 58	-	expression tag
Glu 59	-	expression tag
Glu 60	-	expression tag
Ile 61	-	expression tag
Ile 62	-	expression tag
Glu 63	-	expression tag
Ala 64	-	expression tag
Phe 65	-	expression tag
Val 66	-	expression tag
Gln 67	-	expression tag
Glu 68	-	expression tag
Leu 69	-	expression tag
Arg 70	-	expression tag
Lys 71	-	expression tag
Arg 72	-	expression tag
Gly 73	-	expression tag
Ser 74	-	expression tag
Leu 75	-	expression tag
Val 76	-	expression tag
Pro 77	-	expression tag
Arg 78	-	expression tag
Gly 79	-	expression tag
Ser 80	-	expression tag
Gly 81	-	expression tag
Gly 82	-	expression tag
Pro 99	Ile 99	conflict
Leu 100	Tyr 100	conflict
Val 161	Cys 161	conflict
Ser 165	Glu 165	conflict
Ala 171	Ser 172	conflict
Ile 176	Val 177	conflict
Thr 195	Ser 196	conflict
Ile 204	Val 205	conflict
Phe 354	Tyr 351	conflict
Met 412	Gln 408	conflict
Val 419	Arg 419	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	314515.1
Branched	Number of molecules	12
	Total formula weight	5677.4
Non-Polymers*	Number of molecules	9
	Total formula weight	360.7
All*	Total formula weight	320553.2

*Water molecules are not included.