9O4J
Cryo-EM Structure of the Arabidopsis GA3-GID1A-RGA Complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DELLA protein RGA | polymer | 597 | 65253.3 | 1 | UniProt (Q9SLH3) Pfam (PF12041) Pfam (PF03514) | Arabidopsis thaliana (thale cress) | GAI-related sequence,GRAS family protein 10,AtGRAS-10,Repressor on the ga1-3 mutant,Restoration of growth on ammonia protein 1 |
| 2 | B (B) | Gibberellin receptor GID1A | polymer | 355 | 39775.9 | 1 | UniProt (Q9MAA7) Pfam (PF07859) | Arabidopsis thaliana (thale cress) | AtCXE10,Carboxylesterase 10,GID1-like protein 1,Protein GA INSENSITIVE DWARF 1A,AtGID1A |
| 3 | C (B) | GIBBERELLIN A3 | non-polymer | 346.4 | 1 | Chemie (GA3) |
Sequence modifications
A: 1 - 587 (UniProt: Q9SLH3)
B: 1 - 345 (UniProt: Q9MAA7)
| PDB | External Database | Details |
|---|---|---|
| Gln 163 | Lys 163 | engineered mutation |
| Gln 164 | Arg 164 | engineered mutation |
| Gln 166 | Lys 166 | engineered mutation |
| Gly 588 | - | expression tag |
| Gly 589 | - | expression tag |
| Trp 590 | - | expression tag |
| Ser 591 | - | expression tag |
| His 592 | - | expression tag |
| Pro 593 | - | expression tag |
| Gln 594 | - | expression tag |
| Phe 595 | - | expression tag |
| Glu 596 | - | expression tag |
| Arg 597 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 346 | - | expression tag |
| Gly 347 | - | expression tag |
| Asp 348 | - | expression tag |
| Tyr 349 | - | expression tag |
| Lys 350 | - | expression tag |
| Asp 351 | - | expression tag |
| Asp 352 | - | expression tag |
| Asp 353 | - | expression tag |
| Asp 354 | - | expression tag |
| Lys 355 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 105029.2 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 346.4 | |
| All* | Total formula weight | 105375.5 |
