9O4F

Pre-fusion Stabilized HERV-K Envelope Trimer Ectodomain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Surface protein	polymer	369	42193.4	3	UniProt (Q69384) Pfam (PF13804)	Homo sapiens (human)	SU
2	D, E, F (D, E, F)	Transmembrane protein,Fibritin	polymer	253	27455.5	3	UniProt (Q69384) UniProt (P10104) Pfam (PF00517)	Homo sapiens (human)	TM,Collar protein,Whisker antigen control protein
3	G, L, Q (G, L, Q)	alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		910.8	3	In PDB GlyTouCan (G22768VO)
4	H, J, K, M, O... (H, J, K, M, O...)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	9	In PDB GlyTouCan (G42666HT)
5	BA, CA, I, N, S... (b, c, I, N, S...)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	9	In PDB GlyTouCan (G15407YE)
6	AA, DA, X (a, d, X)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		732.7	3	In PDB GlyTouCan (G32152BH)
7	EA, FA, GA, HA, IA... (A, B, C, D, E...)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

A, B, C: 97 - 465 (UniProt: Q69384)

PDB	External Database	Details
Ile 123	Thr 123	conflict
Arg 159	His 159	conflict
Ser 190	Cys 190	conflict
Ile 328	Val 328	conflict
Val 369	Ile 369	conflict
Cys 437	Val 437	engineered mutation
Ile 449	Val 449	conflict
Arg 463	Ser 463	engineered mutation
Arg 464	Lys 464	engineered mutation

D, E, F: 466 - 632 (UniProt: Q69384)

PDB	External Database	Details
Cys 498	Val 498	engineered mutation
Asp 633	-	linker
Asp 634	-	linker
Asp 635	-	linker
Asp 636	-	linker
Lys 637	-	linker
Ala 638	-	linker
Gly 639	-	linker
Gly 640	-	linker
Ser 641	-	linker
Gly 642	-	linker
Gly 643	-	linker
Ser 644	-	linker
Gly 645	-	linker
Gly 646	-	linker
Ser 647	-	linker
Gly 648	-	linker
Gly 649	-	linker
Gly 650	-	linker

D, E, F: 651 - 676 (UniProt: P10104)

PDB	External Database	Details
Leu 671	Phe 479	conflict
Ala 677	-	expression tag
Ser 678	-	expression tag
Gly 679	-	expression tag
Leu 680	-	expression tag
Glu 681	-	expression tag
Val 682	-	expression tag
Leu 683	-	expression tag
Phe 684	-	expression tag
Gln 685	-	expression tag
Gly 686	-	expression tag
Pro 687	-	expression tag
Gly 688	-	expression tag
Ala 689	-	expression tag
Gly 690	-	expression tag
Trp 691	-	expression tag
Ser 692	-	expression tag
His 693	-	expression tag
Pro 694	-	expression tag
Gln 695	-	expression tag
Phe 696	-	expression tag
Glu 697	-	expression tag
Lys 698	-	expression tag
Gly 699	-	expression tag
Gly 700	-	expression tag
Gly 701	-	expression tag
Ser 702	-	expression tag
Gly 703	-	expression tag
Gly 704	-	expression tag
Gly 705	-	expression tag
Ser 706	-	expression tag
Gly 707	-	expression tag
Gly 708	-	expression tag
Gly 709	-	expression tag
Ser 710	-	expression tag
Trp 711	-	expression tag
Ser 712	-	expression tag
His 713	-	expression tag
Pro 714	-	expression tag
Gln 715	-	expression tag
Phe 716	-	expression tag
Glu 717	-	expression tag
Lys 718	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	208946.8
Branched	Number of molecules	24
	Total formula weight	14029.0
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	224303.1

*Water molecules are not included.