9O34

Replisome (map E)

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (2)	DNA replication licensing factor MCM2	polymer	904	102034.1	1	UniProt (P49736)	Homo sapiens (human)	Minichromosome maintenance protein 2 homolog,Nuclear protein BM28
2	B (3)	DNA replication licensing factor MCM3	polymer	808	91110.9	1	UniProt (P25205)	Homo sapiens (human)	DNA polymerase alpha holoenzyme-associated protein P1,P1-MCM3,RLF subunit beta,p102
3	C (4)	DNA replication licensing factor MCM4	polymer	863	96684.9	1	UniProt (P33991)	Homo sapiens environmental sample	CDC21 homolog,P1-CDC21
4	D (5)	DNA replication licensing factor MCM5	polymer	734	82406.6	1	UniProt (P33992)	Homo sapiens (human)	CDC46 homolog,P1-CDC46
5	E (6)	DNA replication licensing factor MCM6	polymer	821	93010.3	1	UniProt (Q14566)	Homo sapiens (human)	p105MCM
6	F (7)	DNA replication licensing factor MCM7	polymer	719	81411.9	1	UniProt (P33993)	Homo sapiens (human)	CDC47 homolog,P1.1-MCM3
7	G (C)	Cell division control protein 45 homolog	polymer	569	66016.9	1	UniProt (O75419)	Homo sapiens (human)	PORC-PI-1
8	H (D)	DNA replication complex GINS protein PSF1	polymer	196	23022.5	1	UniProt (Q14691)	Homo sapiens (human)	GINS complex subunit 1
9	I (E)	DNA replication complex GINS protein PSF2	polymer	185	21453.7	1	UniProt (Q9Y248)	Homo sapiens (human)	GINS complex subunit 2
10	J (F)	DNA replication complex GINS protein PSF3	polymer	216	24562.6	1	UniProt (Q9BRX5)	Homo sapiens (human)	GINS complex subunit 3
11	K (G)	DNA replication complex GINS protein SLD5	polymer	264	30272.4	1	UniProt (Q9BRT9)	Homo sapiens (human)	GINS complex subunit 4
12	L, M, N (H, I, J)	WD repeat and HMG-box DNA-binding protein 1	polymer	1132	126396.5	3	UniProt (O75717)	Homo sapiens (human)	Acidic nucleoplasmic DNA-binding protein 1,And-1
13	O (K)	Protein timeless homolog	polymer	1211	139118.2	1	UniProt (Q9UNS1)	Homo sapiens (human)	hTIM
14	P (L)	TIMELESS-interacting protein	polymer	301	34600.2	1	UniProt (Q9BVW5)	Homo sapiens (human)
15	Q (M)	Leading Strand DNA	polymer	200	61523.2	1		synthetic construct
16	R (N)	Lagging strand DNA	polymer	182	56347.9	1		synthetic construct
17	S (Q)	Claspin	polymer	1345	152104.6	1	UniProt (Q9HAW4)	Homo sapiens (human)	hClaspin
18	AA, CA, T, W (5, 6, 2, 3)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG) PubChem (888)
19	DA, U, V, Y (6, 2, 3, 5)	PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER	non-polymer		506.2	4	Chemie (ANP)
20	BA, EA, X, Z (6, 7, 4, 5)	ZINC ION	non-polymer		65.4	4	Chemie (ZN) PubChem (32051)

Sequence modifications

C: 1 - 566 (UniProt: O75419)

PDB	External Database	Details
Asp 135	Glu 161	conflict
Tyr 136	-	insertion
Lys 136	-	insertion
Asp 136	-	insertion
Asp 136	Glu 162	conflict
Asp 136	Glu 163	conflict

G: 1 - 223 (UniProt: Q9BRT9)

PDB	External Database	Details
Met -40	-	initiating methionine
Ser -39	-	expression tag
Ala -38	-	expression tag
Trp -37	-	expression tag
Ser -36	-	expression tag
His -35	-	expression tag
Pro -34	-	expression tag
Gln -33	-	expression tag
Phe -32	-	expression tag
Glu -31	-	expression tag
Lys -30	-	expression tag
Gly -29	-	expression tag
Gly -28	-	expression tag
Gly -27	-	expression tag
Ser -26	-	expression tag
Gly -25	-	expression tag
Gly -24	-	expression tag
Gly -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Gly -20	-	expression tag
Ser -19	-	expression tag
Ala -18	-	expression tag
Trp -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
Pro -14	-	expression tag
Gln -13	-	expression tag
Phe -12	-	expression tag
Glu -11	-	expression tag
Lys -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
Leu -7	-	expression tag
Glu -6	-	expression tag
Val -5	-	expression tag
Leu -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag

H, I, J: 1 - 1129 (UniProt: O75717)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

K: 1 - 1209 (UniProt: Q9UNS1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Q: 1 - 1339 (UniProt: Q9HAW4)

PDB	External Database	Details
Glu 1340	-	expression tag
Asn 1341	-	expression tag
Leu 1342	-	expression tag
Tyr 1343	-	expression tag
Phe 1344	-	expression tag
Gln 1345	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	19
	Total formula weight	1534870.2
Non-Polymers*	Number of molecules	12
	Total formula weight	2383.6
All*	Total formula weight	1537253.8

*Water molecules are not included.