9O0G
CryoEM structure of M. mazei topoisomerase VI(A-E342Q)-minicircle DNA complex in cleavage state
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, C) | Type 2 DNA topoisomerase 6 subunit A | polymer | 369 | 42178.3 | 2 | UniProt (Q8PUB7) | Methanosarcina mazei Go1 | Type II DNA topoisomerase VI subunit A |
| 2 | B, D (B, D) | Type 2 DNA topoisomerase 6 subunit B | polymer | 621 | 68853.4 | 2 | UniProt (Q8PUB8) | Methanosarcina mazei Go1 | Type II DNA topoisomerase VI subunit B,TopoVI-B |
| 3 | E (F) | Minicircle DNA sequence | polymer | 35 | 10781.9 | 1 | unidentified | ||
| 4 | F (G) | Minicircle DNA sequence | polymer | 39 | 12011.7 | 1 | unidentified | ||
| 5 | G (H) | Minicircle DNA sequence | polymer | 37 | 11360.3 | 1 | unidentified | ||
| 6 | H (I) | Minicircle DNA sequence | polymer | 37 | 11402.3 | 1 | unidentified | ||
| 7 | I, K, M, O (A, B, C, D) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 8 | J, N (B, D) | PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER | non-polymer | 506.2 | 2 | Chemie (ANP) | |||
| 9 | L, P (B, D) | POTASSIUM ION | non-polymer | 39.1 | 2 | Chemie (K) |
Sequence modifications
A, C: 1 - 369 (UniProt: Q8PUB7)
| PDB | External Database | Details |
|---|---|---|
| Gln 342 | Glu 342 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 267619.5 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1250.9 | |
| All* | Total formula weight | 268870.4 |
