9NYD

Cryo-EM structure of the glycosyltransferase GtrB in the pre-catalysis and product-bound state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Uncharacterized glycosyltransferase sll0501	polymer	341	39319.4	4	UniProt (Q55487) Pfam (PF00535)	Synechocystis sp. PCC 6803 substr. Kazusa
2	E, H, N (A, B, D)	URIDINE-5'-DIPHOSPHATE-GLUCOSE	non-polymer		566.3	3	Chemie (UPG)
3	F, I, O (A, B, D)	[(2~{E},6~{E},10~{E},14~{E},18~{Z},22~{E},26~{Z},30~{E},34~{E},38~{E})-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,14,18,22,26,30,34,38,42-undecaenyl] dihydrogen phosphate	non-polymer		847.3	3	Chemie (5TR)
4	G, J, K, P (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
5	L (C)	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	1	Chemie (UDP)
6	M (C)	undecaprenyl phosphate-glucose	non-polymer		1009.4	1	Chemie (A1B94)

Sequence modifications

A, B, C, D: 2 - 318 (UniProt: Q55487)

PDB	External Database	Details
Met -22	-	initiating methionine
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Ser -15	-	expression tag
Ser -14	-	expression tag
Gly -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Leu -10	-	expression tag
Gly -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Ser -1	-	expression tag
Asn 0	-	expression tag
Ala 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	157277.7
Non-Polymers*	Number of molecules	12
	Total formula weight	5751.6
All*	Total formula weight	163029.3

*Water molecules are not included.