9NY4

USP21 bound to H2AK119ub nucleosome

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	136	15435.1	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (African clawed frog)	Histone H3
2	B, F (B, F)	Histone H4	polymer	103	11394.4	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (African clawed frog)
3	C, G (C, G)	Histone H2A type 1	polymer	130	14083.4	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211)	Xenopus laevis (African clawed frog)
4	D, H (D, H)	Histone H2B 1.1	polymer	123	13629.9	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (African clawed frog)	H2B1.1
5	I (I)	601 DNA (145-MER)	polymer	145	44520.4	1		synthetic construct
6	J (J)	601 DNA (145-MER)	polymer	145	44991.7	1		synthetic construct
7	K (K)	Ubiquitin carboxyl-terminal hydrolase 21	polymer	370	41744.6	1	UniProt (Q9UK80) Pfam (PF00443)	Homo sapiens (human)	Deubiquitinating enzyme 21,Ubiquitin thioesterase 21,Ubiquitin-specific-processing protease 21
8	L (U)	Ubiquitin	polymer	80	9036.4	1	UniProt (J3QS39) Pfam (PF00240)	Homo sapiens (human)

Sequence modifications

A, E: 0 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	engineered mutation

C, G: 0 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	conflict
Cys 119	Lys 120	conflict
Ser 123	Ala 124	conflict

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Met 0	-	expression tag
Thr 29	Ser 33	engineered mutation
Cys 117	Lys 121	engineered mutation

U: 1 - 76 (UniProt: J3QS39)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	249378.7
All*	Total formula weight	249378.7

*Water molecules are not included.