9NXE

Crystal Structure of Calcineurin Clinical Variant E282K

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein phosphatase 3 catalytic subunit alpha	polymer	370	42659.9	1	UniProt (Q08209) Pfam (PF00149)	Homo sapiens (human)	CAM-PRP catalytic subunit,Calcineurin A alpha,Calmodulin-dependent calcineurin A subunit alpha isoform,CNA alpha,Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
2	B (B)	Calcineurin subunit B type 1	polymer	156	17755.2	1	UniProt (P63098) Pfam (PF13499)	Homo sapiens (human)	Protein phosphatase 2B regulatory subunit 1,Protein phosphatase 3 regulatory subunit B alpha isoform 1
3	C (C)	Sodium/hydrogen exchanger 1	polymer	48	5268.0	1	UniProt (P19634)	Homo sapiens (human)	APNH,Na(+)/H(+) antiporter,amiloride-sensitive,Na(+)/H(+) exchanger 1,NHE-1,Solute carrier family 9 member 1
4	D (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	E (A)	FE (III) ION	non-polymer		55.8	1	Chemie (FE)
6	F (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
7	G, H, I, J (B)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
8	K, L, M (A, B, C)	water	water		18.0	233	Chemie (HOH)

Sequence modifications

A: 1 - 370 (UniProt: Q08209)

PDB	External Database	Details
Lys 282	Glu 282	engineered mutation

B: 16 - 170 (UniProt: P63098)

PDB	External Database	Details
Met 15	-	expression tag

C: 679 - 723 (UniProt: P19634)

PDB	External Database	Details
Gly 676	-	expression tag
His 677	-	expression tag
Met 678	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	65683.1
Non-Polymers*	Number of molecules	7
	Total formula weight	387.7
All*	Total formula weight	66070.8

*Water molecules are not included.