9NWE

E3 ligase UBR4-KCMF1-calmodulin complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	E3 ubiquitin-protein ligase UBR4	polymer	5205	577180.9	2	UniProt (Q5T4S7) Pfam (PF19423) Pfam (PF02207) Pfam (PF13764)	Homo sapiens (human)	600 kDa retinoblastoma protein-associated factor,p600,N-recognin-4,Retinoblastoma-associated factor of 600 kDa,RBAF600
2	C, D (C, D)	Calmodulin-1	polymer	149	16852.5	2	UniProt (P0DP23) Pfam (PF13499)	Homo sapiens (human)
3	E, F (E, F)	E3 ubiquitin-protein ligase KCMF1	polymer	381	41992.3	2	UniProt (Q9P0J7) Pfam (PF00569) Pfam (PF05605)	Homo sapiens (human)	FGF-induced in gastric cancer,Potassium channel modulatory factor,PCMF,ZZ-type zinc finger-containing protein 1
4	G, H, I, J, K... (A, B)	ZINC ION	non-polymer		65.4	6	Chemie (ZN)
5	M, N, O, P (C, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)

Sequence modifications

A, B: 2 - 5183 (UniProt: Q5T4S7)

PDB	External Database	Details
Met -21	-	initiating methionine
Asp -20	-	expression tag
Tyr -19	-	expression tag
Lys -18	-	expression tag
Asp -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Gly -14	-	expression tag
Asp -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Asp -10	-	expression tag
His -9	-	expression tag
Asp -8	-	expression tag
Ile -7	-	expression tag
Asp -6	-	expression tag
Tyr -5	-	expression tag
Lys -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Asp 0	-	expression tag
Lys 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	1272051.7
Non-Polymers*	Number of molecules	10
	Total formula weight	552.8
All*	Total formula weight	1272604.4

*Water molecules are not included.